Projects and Grants per year
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Research Interests
My research interests are centered on the role(s) of redox state and cancer progression, particularly, subcellular redox states. Elucidating the molecular mechanisms underlying redox state-regulated gene/protein expression is critical for understanding the behavior of cancer cells.
Previously, we discovered that both intra- and extracellular redox states were significantly different between prostate cancer cells and normal prostate epithelial cells; the intracellular redox state becomes more oxidized while the extracellular redox state becomes more reduced in prostate cancer cell lines. These differences correlated with prostate cancer cell growth and invasion.
Additionally, alteration of extracellular redox state by overexpression of extracellular superoxide dismutase (ECSOD) or decreasing of extracellular spaces redox potential values through varying cysteine/cystine ratio significantly inhibited prostate cancer invasion, whereas alteration of intracellular redox state by overexpression of manganese superoxide dismutase (MnSOD) or treatment with glutathione-depleting compounds significantly inhibited cell growth.
Currently, we investigate the expression levels of selected antioxidant proteins (e.g. MnSOD, thioredoxin 1, or ECSOD) at specific subcellular organelle in epithelial vs. stromal cells of benign vs. cancer tissues of prostate cancer patients using immunohistochemistry staining with specific antibodies and Aperio Image analysis system. The study is the first to focus on site-specific intra- and extracellular redox imbalances as new therapeutic target proteins for prostate cancer treatment. Another important and exciting aspect of my current work is using the cutting-edge tool, nitroxides-enhanced MRI to measure tissue redox activity in prostate cancer of living mice. The assay is based on nitroxides redox cycling coupled with the appearance/disappearance of an enhanced MRI signal, which makes them useful molecular sensors for changing redox state. This method may be used as a tool for diagnosis and therapy guidance since it has low toxicity, minimal side effects, and has a short half-life for imaging.
In summary, differences in tissue redox activities and antioxidant protein levels may serve as biomarkers to identify cancer from non-cancer in prostate biopsies and identify patients with low-grade cancers who will progress to high-grade cancers, providing possible new information to guide therapeutic decisions.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Education/Academic qualification
Post Doctoral Scholar, University Of Wisconsin-Madison
2009
Doctor of Philosophy, Mahidol University
2004
Bachelor of Science, Mahidol University
1999
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Collaborations and top research areas from the last five years
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CCSG Pilot: Targeting Mitochondria in Prostate Cancer with Novel A Gold (Au)-based Therapeutic Compound to Improve Radiation Treatment
Chaiswing, L. (PI) & Awuah, S. (CoI)
2/1/25 → 1/31/26
Project: Research project
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IPA: Modulation of Mitochondrial Respiration to Treat Colitis
Chaiswing, L. (PI)
8/1/24 → 12/31/25
Project: Research project
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Diversity Supplement for Kahleel Guerrier: Targeting Mitochondrial Redox Capacity to Overcome Cancer Subtype that Regrowth After Radiation
Chaiswing, L. (PI) & St Clair, D. (CoI)
8/1/23 → 3/31/26
Project: Research project
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Targeting Mitochondrial Redox Capacity to Overcome Cancer Subtype that Regrowth After Radiation
Chaiswing, L. (PI), Higashi, R. (CoI), Luo, W. (CoI), St Clair, W. (CoI) & Weiss, H. (CoI)
4/1/21 → 3/31/26
Project: Research project
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Extracellular vesicles released by ALL patients contain HNE-adducted proteins: Implications of collateral damage
Ho, J., Sukati, S., Taylor, T., Carter, S., Fuller, B., Marmo, A., Sorge, C., D'Orazio, J., Butterfield, D. A., Bondada, S., Weiss, H., St Clair, D. K. & Chaiswing, L., Feb 1 2025, In: Free Radical Biology and Medicine. 227, p. 312-321 10 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Glycosaminoglycan modification of NRP1 exon 4-skipping variant drives colorectal cancer metastasis via endosomal-exosomal trafficking
Gu, Y., Ye, Q., Huang, X., Cao, Y., Chaiswing, L. & She, Q. B., Jun 28 2025, In: Cancer Letters. 620, 217683.Research output: Contribution to journal › Article › peer-review
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Correction to: Extraction of redox extracellular vesicles using exclusion-based sample preparation (Analytical and Bioanalytical Chemistry, (2024), 416, 28, (6317-6331), 10.1007/s00216-024-05518-z)
Banadaki, M. D., Rummel, N. G., Backus, S., Butterfield, D. A., St. Clair, D. K., Campbell, J. M., Zhong, W., Mayer, K., Berry, S. M. & Chaiswing, L., Dec 2024, In: Analytical and Bioanalytical Chemistry. 416, 29, p. 7187-7188 2 p.Research output: Contribution to journal › Comment/debate
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Extraction of redox extracellular vesicles using exclusion-based sample preparation
Banadaki, M. D., Rummel, N. G., Backus, S., Butterfield, D. A., St. Clair, D. K., Campbell, J. M., Zhong, W., Mayer, K., Berry, S. M. & Chaiswing, L., Nov 2024, In: Analytical and Bioanalytical Chemistry. 416, 28, p. 6317-6331 15 p.Research output: Contribution to journal › Article › peer-review
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A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP5+, Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line
Chaiswing, L., Yarana, C., St. Clair, W., Tovmasyan, A., Batinic-Haberle, I., Spasojevic, I. & St. Clair, D., 2022, In: Oxidative Medicine and Cellular Longevity. 2022, 9664636.Research output: Contribution to journal › Article › peer-review
Open Access10 Scopus citations