Grants and Contracts Details
Description
A Cell-Based Biosensor for Earlv Detection of Cancer
Early detection of cancer and an understanding of the level of aggressiveness are keys to
successful treatment of the disease. Current clinical efforts to detect cancer and its
aggressiveness are hindered because they usually involve invasive procedures. Recent
research efforts have focused on non-invasive techniques for early detection through
analysis of agents (e.g. VGEF, IGF-l) released in serum ofthe patient. A methodology,
however, for using these agents for early detection has not been achieved. We have
recently developed a cell-based biosensor that can be used to detect small concentrations
of various agents in solution. The biosensor consists of a monolayer of endothelial cells
attached to the membrane of an ion-selective electrode (ISE). When the cells are
attached to the surface and form the monolayer, tight junctions form between the cells
inhibiting the response of the ISE to ions in solution. When the monolayer is exposed to
agents that affect the permeability of the endothelial cells, the ions can diffuse through
the membrane and a potential response ITom the ISE is achieved. In addition, the
measured potential ITomthe electrode depends on the concentration of the permeabilitymodifying
agent and hence, a relationship between the potential value and concentration
of the agent can be achieved. This proposed study will develop the technology for using
this sensor in detecting agents that are indicative of early stages of cancer and will
optimize the response to predict the aggressiveness of the cancer. In addition, the
miniaturization of the sensor will be pursued. These studies will provide the basis for
future studies where these sensors will be used to test for agents in serum of tumorbearing
nude mice and patients. Ultimately, this study will lead to the development of a
cost-effective, non-invasive procedure for early detection of cancer and screening for
level of aggressiveness.
List ofKev Words:
Cell-Based Biosensor
Cancer
Early Detection
Ion Selective Electrode
Endothelial Cells
6
Status | Finished |
---|---|
Effective start/end date | 11/1/05 → 4/30/07 |
Funding
- KY Science and Technology Co Inc: $49,996.00
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