ABTR04B1 Establishing Continuous Cell Lines and Xenografts from Pediatric Cancers for Biological and Pre-Clinical Therapeutic Studies

Grants and Contracts Details

Description

Biological studies of tumor and cell specimens from childhood cancer are limited by the size of the specimens, which must be shared among a number of investigators. Carefully generated cell lines provide a well-established means of generating a renewable source of tumor cells for cancer biology studies. Cells growing in culture also allow studies to be done that are not otherwise possible with fixed or frozen tissue, such as testing of new drugs for anti-cancer properties and conducting experiments in which the biological effects of molecular pathways are validated by gene “knock-in” or “knock-out” approaches or the use of specific inhibitors of gene function. This protocol will utilize tumors and malignant cells collected under existing COG therapeutic, biological, and banking protocols to generate continuously growing cell lines and/or xenografts in immunocompromised mice. The protocol will only utilize excess patient material after all disease-committee therapeutic and biological study requirements have been satisfied. As it is anticipated that for acute lymphoblastic leukemia (ALL), direct expansion of cells in nod/SCID mice will be more successful than cell culture, the priority for ALL cells submitted under this protocol will be to attempt mouse xenografts. Cell lines or xenografts will be banked in the form of multiple reference vials containing cryopreserved cells with careful and rigorous quality controls. Selected cell lines will be characterized for relevant biological features by disease committee-designated laboratories, or by the cell culture laboratory in close collaboration with disease committee investigators. A panel of selected cell lines will be designated by each disease committee for expansion and that expanded cell line repository will be banked at Texas Tech University Health Sciences Center (TTUHSC) for distribution to COG-approved investigators. A database of cell lines in the repository, their known biological characteristics, and publications using or describing the cell lines will be maintained on the COG web site. It is anticipated that the cell line repository that will be created by this protocol will provide a unique and highly useful, renewable resource of biological specimens for cancer research.
StatusActive
Effective start/end date3/1/212/28/25

Funding

  • Public Health Institute: $2.00

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.