Grants and Contracts Details
Description
Periodontitis represents the most broadly distributed polymicrobial disease of mankind. The tissue destructive
processes that lead to loss of function of periodontal soft tissues and alveolar bone result from a chronic
polymicrobial biofilm activation of host inflammatory, innate immune, and adaptive immune responses in the
local tissues. Development of the polymicrobial biofilm is a sequential process that begins with deposition of
host and bacterial glycoproteins onto the clean tooth surface. Initial colonization by oral microorganisms
occurs next, with oral streptococci being the primary early colonizers. This initial colonization by streptococci is
followed by increasing proportions of Actinomyces, Veillonella, and Fusobacterium spp. (middle colonizers)
with subsequent colonization predominated by Gram-negative, anaerobic bacteria (late colonizers). This shift
from a predominance of Gram-positive, facultative microorganisms to a microbiota dominated by Gramnegative,
anaerobic species is manifest clinically by inflammatory changes in the periodontal tissues as the
host responds to this microbial challenge. It has been proposed that this multicellular bacterial-host interaction
possesses unique features compared to planktonic bacterial infectious challenges; however, there is little
objective evidence to support this contention. This R21 exploratory/development grant will specifically focus
on developing this evidence using a novel polymicrobial biofilm model. The General Hvpotheses to be tested
are: (1) Oral bacteria in biofilms will elicit different patterns of host responses from gingival epithelial cells; and
(2) Host cells will respond differently to bacterial species present as monospecies versus multispecies biofilms.
We have developed a novel in vitro biofilm-host cell model system using a rigid, gas permeable contact lens
material. In Specific Aim 1 we will determine the characteristics of single species and multispecies biofilms
created on this material. The biofilms developed in this model system will then be used in Specific Aim 2 to
challenge oral epithelial cells, allowing us to characterize the patterns of responses elicited in these host cells
by the bacterial challenge. In accomplishinQ these aims. we will have developed a novel approach for
evaluatin host res onse atterns to lanktonic sin Ie s ecies biofilm and 01 microbial biofilm challen es.
The results obtained will enable us to provide seminal data to enable a further exploration of host responses to
biofilms of various compositions and complexities.
Status | Finished |
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Effective start/end date | 3/1/08 → 2/28/11 |
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