Grants and Contracts Details
Description
PROJECT SUMMARY (See instructions):
Parent Grant Specific Aim 2: To reduce activation in visceronociceptive pathways that maintain central
sensitization induced by a persistent experimental pancreatitis.
New Experiment (Extension of the original)
We propose in vitro studies to examine protective and anti-inflammatory effects of met-enkephalin. Rats will
be fed a high fat and alcohol diet that produces pancreatitis and activates the pancreatic stellate cells. We will
harvest and then examine the stellate cells in primary cultures, as well as assess treatments in clonal PANC-1
cells. The activators will include alcohol and arachidonic acid. Each of the in vitro studies proposed will compare
averaged responses of treated cells in a minimum of four experiments done in triplicate. Three doses of the
alcohol/inflammation activators will be tested.
Group I Untreated cells
Group II Alcohol treated cells (50, 150, 500mM)
Group Ill Alcohol treated cells + met-enkephalin
Group IV Arachidonic acid treated cells (10, 30, lOO1tM)
Group V Arachidonic acid treated cells + met-enkephalin
N=3 in triplicate for each. Each group will be compared to untreated cells using Student t-tests (p
Status | Finished |
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Effective start/end date | 4/1/00 → 10/31/10 |
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