Assessing the Health Effects of Blast Injuries and Embedded Metal Fragments

STATEMENT OF WORK PROPOSED START DATE June 01, 2016 60 Month Period of Performance Project 2: Biomarkers for Assessing Return-to-Duty Potential of Personnel with Embedded Metal-Fragment Wounds PI: Charlotte A. Peterson, PhD University of Kentucky, College of Health Sciences 900 South Limestone, KY 40536 Task Timeline Year - Month Major Task 1 – Experimental Preparation Subtask 1: Establish financial accounts 1-1 to 1-2 Subtask 2: Initiate hiring process for Research Scientist II 1-4 to 1-6 Subtask 3: Order required supplies for RNA & exosome isolation 1-6 to 1-8 Subtask 4: Hire and train Research Scientist II 1-7 to 1-10 Subtask 5: Optimize RNA & exosome isolation, quality control assessment 1-10 to 1-12 Milestones Achieved: Major Task 1 completed 1-12 Major Task 2 – 3M experimental group microarray analyses Subtask 1: Receive 3M group urine samples 2-4 Subtask 2: Receive 3M group muscle and serum samples 2-4 Subtask 3: Isolate total RNA from 3M group muscle samples 2-5 to 2-7 Subtask 4: Isolate exosomes & RNA from 3M group urine & serum samples 2-8 to 2-10 Subtask 5: Quality assessment of RNA samples 2-11 Subtask 6: Gene and microRNA microarray experiments 2-12 to 3-3 Subtask 7: Identification of differentially expressed genes & microRNAs 3-4 to 3-6 Subtask 8: RT-PCR validation of differentially expressed genes & microRNAs 3-7 to 3-9 Subtask 9: Identify predicted microRNA target genes 3-10 Milestones Achieved: Major Task 2 Completed 3-10 Task Timeline Year - Month Major Task 3 – 12M experimental group microarray analyses Subtask 1: Receive 12M group urine samples 2-8 Subtask 2: Receive 12M group muscle and serum samples 2-8 Subtask 3: Isolate total RNA from 12M group muscle samples 2-9 to 2-12 Subtask 4: Isolate exosomes & RNA from 12M group urine & serum samples 3-1 to 3-3 Subtask 5: Quality assessment of RNA samples 3-4 Subtask 6: Gene and microRNA microarray experiments 3-5 to 3-9 Subtask 7: Identification of differentially expressed genes & microRNAs 3-10 to 3-12 Subtask 8: RT-PCR validation of differentially expressed genes & microRNAs 4-1 to 4-3 Subtask 9: Identify predicted microRNA target genes 4-4 Milestones Achieved: Major Task 3 Completed 4-4 Major Task 4 – 6M experimental group microarray analyses Subtask 1: Receive 6M group urine samples 3-5 Subtask 2: Receive 6M group muscle and serum samples 3-5 Subtask 3: Isolate total RNA from 6M group muscle samples 3-6 to 3-7 Subtask 4: Isolate exosomes & RNA from 6M group urine & serum samples 3-8 to 3-10 Subtask 5: Quality assessment of RNA samples 3-11 Subtask 6: Gene and microRNA microarray experiments 3-12 to 4-3 Subtask 7: Identification of differentially expressed genes & microRNAs 4-4 to 4-6 Subtask 8: RT-PCR validation of differentially expressed genes & microRNAs 4-7 to 4-9 Subtask 9: Identify predicted microRNA target genes 4-10 Milestones Achieved: Major Task 4 Completed 4-10 Major Task 5 – 1M experimental group microarray analyses Subtask 1: Receive 1M group urine samples 3-9 Subtask 2: Receive 1M group muscle and serum samples 3-9 Subtask 3: Isolate total RNA from 1M group muscle samples 3-10 to 3-11 Subtask 4: Isolate exosomes & RNA from 1M group urine & serum samples 3-12 to 4-2 Subtask 5: Quality assessment of RNA samples 4-3 Subtask 6: Gene and microRNA microarray experiments 4-4 to 4-8 Subtask 7: Identification of differentially expressed genes & microRNAs 4-9 to 4-12 Subtask 8: RT-PCR validation of differentially expressed genes & microRNAs 5-1 to 5-3 Subtask 9: Identify predicted microRNA target genes 5-4 Milestones Achieved: Major Task 5 Completed 5-4 Major Task 6 – Data Compilation, Statistical Analysis, and Preparation of Final Report Subtask 1: Data compilation and statistical analysis of all microarray data 5-5 to 5-9 Subtask 2: Development of gene regulatory network based on differentially expressed gene and microRNAs 5-10 Subtask 3: Begin preparation of final report 5-11 to 5-12 Subtask 4: Complete and submit final report to Program Officer 5-12 Milestones Achieved: Major Task 6 Completed 5-12