Characterization of Microglial P2X Signaling and Phenotypes in Alzheimer's Patients

Dr. Bachstetter’s lab will be responsible for staining human brain tissue using the 3rd generation in situ hybridization chain reaction (HCR) probes developed by Dr. Monaghan’s lab. He will provide Dr. Monaghan deidentified human brain tissue from brain bank to Dr. Monaghan for use in HCR probe development. Dr. Bachstetter’s lab will be responsible for assessing the localization of the HCR probes in relation to the microglia morphology in Alzheimer’s disease and control brain cases. Dr. Bachstetter’s lab will also provide the Keekenes-Huskey lab with digital microscope slides of brain tissue stained with microglia markers to be used for the automated computer vision techniques to classify subcellular morphologies in intact tissue. Finally, Dr. Bachstetter will provide expertise in Alzheimer’s disease and microglia biology for the interpretation and reporting of the findings of the project.