Projects and Grants per year
Grants and Contracts Details
Description
We will test our hypotheses with the following specific aims:
1) Quantify MRI-based measures of systolic and diastolic function in diet-induced obese mice
compared to control mice serially throughout 16 weeks of feeding. Non-invasive quantification of
myocardial function from cardiac MRI in mouse models has only recently become possible with
improvements in the efficiency of image acquisition sequences and MRI field strengths (Figure 2).10 We
propose to collaborate with a world leader in rodent cardiac MRI at the University of Virginia, Dr. Frederick
Epstein, to quantify these advanced measures of cardiac function in mouse models of diet-induced obesity
and control mice. The University of Kentucky recently purchased a state-of-the art Bruker 7 Tesla Clinscan
MRI identical to the scanner that Dr. Epstein uses, thus facilitating a strong inter-institutional collaboration.
2) Determine the accuracy of T1-mapping MRI to quantify diffuse cardiac fibrosis compared to
histology in mice who have received angiotensin infusion for 4 weeks. Recent advances in MRI
technology have enabled acquisition of pre and post-contrast T1-relaxation time maps of the myocardium.
Parameters derived from these T1 maps are thought to be related to levels of diffuse fibrosis in several
clinical studies.11-13 However, this methodology has not been validated against histology in mouse models
known to have diffuse fibrosis such as the proposed angiotensin infusion model.
3) Measure the accuracy of proton magnetic resonance spectroscopy to quantify intramyocardial lipid
content compared to post-mortem mass spectrometry in leptin-deficient ob/ob and control mice.
Magnetic resonance spectroscopy has been used in several clinical studies to quantify intracellular lipid
content in cardiac myocytes.6, 14, 15 However, only one study has attempted to validate this technique in
mice16, and the results are limited by the fact that the authors did not see a correlation between triglyceride
content and lipid content measured with imaging. This is likely due to the fact that the authors only
measured triglyceride content, while we propose to quantify the full spectrum of lipids using state-of-the-art
mass spectrometry available at our institution
Status | Finished |
---|---|
Effective start/end date | 9/8/08 → 9/14/13 |
Funding
- National Institute of General Medical Sciences
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Projects
- 1 Finished
-
COBRE Administrative Core A: Center of Research in Obesity and Cardiovascular Disease
Cassis, L., Charnigo, R., Daugherty, A., Kern, P., Li, Z., Morris, A., Pearson, K., Randall, D., Smyth, S., Van Der Westhuyzen, D., Webb, N., Zhou, C. & de Beer, F.
National Institute of General Medical Sciences
9/8/08 → 9/14/13
Project: Research project