Collaborative Research: CPSF30 at the convergence of cellular signaling and RNA Processing

Grants and Contracts Details


The subject of this proposal is a hypothetical hub that transduces cellular signaling cues into differential poly(A) site choices and phenotypic outcomes. This hub, the 30 kD subunit of the so-called Cleavage and Polyadenylation Specificity Factor in Arabidopsis (AtCPSF30), is regulated in vitro by two distinctive chemical or biochemical signals (calcium and redox signals), and is involved in a range of developmental and physiological responses. The goals of the research proposed in this submission are to determine how these different signaling cues are transduced to specific outcomes. The goals will be accomplished by testing these three hypotheses: 1. Different signaling inputs are processed, through AtCPSF30, into differing sets of APA events and outcomes. 2. AtCPSF30-mediated APA alters mRNA functionality for some of the numerous direct targets of the protein. 3. AtCPSF30-mediated APA affects gene expression early in lateral root development. Extensive use will be made of systems approaches - both high-throughput DNA sequencing and computational analyses of large datasets. These will be complemented by genetic, transgenic, cellular, molecular, and biochemical studies, all of which will be brought to bear on tests of the stated hypotheses.
Effective start/end date9/1/148/31/18


  • National Science Foundation: $303,269.00


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