Developing RNAi for Suppression of Exotic Wood-Boring Buprestids

Abstract RNAi-Buprestid We are developing techniques to utilize an innovative pest suppression approach, RNA interference (RNAi), to target wood-boring Buprestidae, focusing on aggressive, non-native species. RNAi is a gene-silencing mechanism that is an emerging approach to insect pest management. This interference manifests itself in compromised development, reduced fecundity, or direct mortality. RNAi is highly specific to the target species, and there is considerable variability in the efficacy of RNAi between different species. We’ve discovered that RNAi works and can kill the aggressive, invasive emerald ash borer (EAB). Optimal gene selection is progressing, and this could be a promising approach for closely related species. However, demonstrating the specificity of the selected genes, and developing appropriate delivery methods of the dsRNA to the target is essential for implementation of RNAi as a management approach. These are the objectives of this proposal.