Development of an On-site Diagnostic Device for Johne's Disease

  • Branscum, Adam (PI)

Grants and Contracts Details


Jobne's disease (3D), caused by Mycobacterium avium subsp. paratuberculosis (MAP), has a significant economic impact on the US dairy and beef cattle industries and yet current diagnostic tests are of limited success For example, ELISAs have relatively low sensitivity for MAP, detecting only 30%. The fecal culture and PCR tests are relatively expensive and time-consuming. We developed recently a novel Ethanol-Vortex-Absorption ELISA (EVA-ELISA) which showed high levels of empirical diagnostic sensitivity (98.5%) and specificity (100%). However, all of the current diagnostic tests, including the EVA-ELISA, need to be conducted in diagnostic laboratories requiring a labor-intensive process involving collecting samples into proper containers, indexing, packing, and shipping. There is, therefore, a need for the development of a novel diagnostic test for 3D that can be conducted in field and is capable of detecting the disease with high sensitivity. We propose to develop a highly sensitive, on-site diagnostic test for 3D by combining the MA? surface antigens Lised in the EVA-ELISA and a state-of-the-art technology, microfluidics. The specific objective of this proposal is to demonstrate a `proof-of-concept~ that bovine 3D can be diagnosed by employing a test based on the nicrofluidic technology.
Effective start/end date8/8/0810/31/09


Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.