Grants and Contracts Details
Description
Jobne's disease (3D), caused by Mycobacterium avium subsp. paratuberculosis (MAP), has a significant
economic impact on the US dairy and beef cattle industries and yet current diagnostic tests are of limited success
For example, ELISAs have relatively low sensitivity for MAP, detecting only 30%. The fecal culture and PCR
tests are relatively expensive and time-consuming. We developed recently a novel Ethanol-Vortex-Absorption
ELISA (EVA-ELISA) which showed high levels of empirical diagnostic sensitivity (98.5%) and specificity
(100%). However, all of the current diagnostic tests, including the EVA-ELISA, need to be conducted in
diagnostic laboratories requiring a labor-intensive process involving collecting samples into proper containers,
indexing, packing, and shipping. There is, therefore, a need for the development of a novel diagnostic test for
3D that can be conducted in field and is capable of detecting the disease with high sensitivity.
We propose to develop a highly sensitive, on-site diagnostic test for 3D by combining the MA? surface antigens
Lised in the EVA-ELISA and a state-of-the-art technology, microfluidics. The specific objective of this proposal
is to demonstrate a `proof-of-concept~ that bovine 3D can be diagnosed by employing a test based on the
nicrofluidic technology.
Status | Finished |
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Effective start/end date | 8/8/08 → 10/31/09 |
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