Effects of Genetics on the Expectation and Experience of Orthodontic Pain

Scope of Work for Agreement for the University of Kentucky (UK) Center for the Biologic Basis of Oral/Systemic Diseases, Genetics/Genomics Core (Dr. James Hartsfield and Dr. Lorri Morford) with the University of Florida for project entitled: “Effects of Genetics on the Expectation and Experience of Orthodontic Pain.” The University of Kentucky (Dr. James K. Hartsfield and Dr. Lorri Ann Morford) will: 1) The UK Genetics/Genomics Core will receive saliva samples from the University of Florida (Dr. John Neubert) in specific buffered preservative containers (sold by DNAgenotek, Inc., Ontario, Canada). These containers will be labeled with an anonymous identifying code given by the University of Florida (Dr. John Neubert) for the purpose of data acquisition and analysis. 2) The UK Genetics/Genomics Core (Dr. Morford) will extract genomic DNA from the coded saliva samples in the Biologic Basis of Oral/Systemic Diseases Hereditary Genetics/Genomics Core Laboratory (MN279) using the standard methodology recommended by DNAgenotek. 3) The UK Genetics/Genomics Core (Dr. Morford) will perform TaqMan® single nucleotidepolymorphism (SNP) genotyping for SNPs specified in the research protocol. 4) The UK Genetics/Genomics Core (Dr. Hartsfield) will perform statistical analysis of the SNP data with the anonymously coded data on the subjects from the University of Florida (Dr. John Neubert). 5) The UK Genetics/Genomics Core will report the basic data and statistical analysis results to the University of Florida (Dr. John Neubert) in writing delivered via email (or hardcopy by mail or 1-2-day carrier). 6) Drs. Hartsfield and Morford will participate in the writing, editing and submission of any manuscript that may result from this activity.