Grants and Contracts Details
Description
~tharoicImU laths n*r sinai aaftnaty heart noses and sVoS. ~nrc ~
as well as envi-onntntal factors contribute to tf~s disease. A recent collabcwston
etween our lab and the lab of [Jr. Jalne Lusis at UCLA has revealed that Zinc
ringers and horreoboxea 2 (Zhx2) is the gene responsible for the
hyperipidenia/atherosclerosis phenotype defined by t-lyplip2 (Accepted,
)rculation: Cardiovascular Geneucs). Furthermore, studies it, our lab and lhe
Lusis lab have shown that several hepat enzymes involved in ipid homeostasis
are targets of Zhx2 regulation. Thus, a greater understanding of the mechanism by
hich Zhx2 regulates target genes nay shed light on lhe genetic influence on
atherosclerosis and is therefore of substantal clinical significance.
Thx2 was ideritfied in our lab by positional cloning- Zhx2 functions ass repressor
slpha-fetoprotein ~AFP) and H19 gene expression in the aduk liver. Our recent
data show that Zhx2 does not ater the transcription rate across lhe AFngene but
rather, represses AFP nRNLA accuntilaton by inhibiting spicing of several APP
`eons, in a pron'oter dependent mnanner `hypothesize that Zhx2 acts through the
pronoter of its target genes to affect the splicing of nascent Rt~, thus post-
renscriptionally dow n-regulating the level of fully processed rrfif-IA. To test this -
iypotheais and to understand this noveL mechanism of gene regulation, I propose
lidentify AFP promoter sequence and Zhx2 protein domains required fer
Status | Finished |
---|---|
Effective start/end date | 7/1/10 → 6/30/12 |
Funding
- American Heart Association: $46,000.00
Fingerprint
Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.