Grants and Contracts Details
Description
Progress report:
We performed experiments to determine if DNA methylation is related to
resistance to CYP1A induction in Newark Bay (NJ) killifish. Newark Bay killifish
embryos were exposed to the DNA de-methylating agent Aza-C in combination
with 3,3'4,4',5-pentachlorobiphenyl (PeCB). Aza-C treatmenty did not result in
CYP1A induction by PeCB in the Newark Bay, PCB resistant killifish.
We also used a restriction enzyme approach to measure DNA methylaiton
of the Newark Bay killifish CYP1A gene promoter. This approach did not reveal
any difference in DNA methylation between PCB resistant (Newark Bay) and
PCB responsive (reference site) killifish. Our results suggest that methylation is
not involved in the resistant phenotype posed by the Newark Bay killifish.
Previous DNA sequence analysis of the killifish CYP1A promoter revealed
a 50 base pairs insertion that was present in some promoters but not in others.
We studied the distribution of these inserts in the Newark Bay and Flax Pond
(reference site) killifish populations using a PCR approach. Our results suggest
that these insertions are not present in any of the Newark Bay killifish, but it
occurred frequently on the promoters of Flax Pond killifish.
Presentations at professional meetings:
1. 2002 Society of Environmental Toxicology and Chemistry 23rdAnnual
Meeting. Sequence Comparison of the CYP1A promoter of AHR ligand resistant
and responsive killifish (Fundulus heteroclitus). Arzuaga, X. and Elskus, A.
2. 2003 Pollutant Response in Marine Organisms 1ih International Symposium.
Resistance to CYP1A induction in Newark Bay killifish (Fundulus heteroclitus) is
not due to DNA methylation. Arzuaga, X., Calcano, W., and Elskus, A.
Status | Finished |
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Effective start/end date | 9/26/02 → 9/25/04 |
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