Fellowship Xabier Arzuaga: Molecular Mechanisms of Resistance in Vertebrates

  • Arzuaga, Xabier (CoI)

Grants and Contracts Details


Progress report: We performed experiments to determine if DNA methylation is related to resistance to CYP1A induction in Newark Bay (NJ) killifish. Newark Bay killifish embryos were exposed to the DNA de-methylating agent Aza-C in combination with 3,3'4,4',5-pentachlorobiphenyl (PeCB). Aza-C treatmenty did not result in CYP1A induction by PeCB in the Newark Bay, PCB resistant killifish. We also used a restriction enzyme approach to measure DNA methylaiton of the Newark Bay killifish CYP1A gene promoter. This approach did not reveal any difference in DNA methylation between PCB resistant (Newark Bay) and PCB responsive (reference site) killifish. Our results suggest that methylation is not involved in the resistant phenotype posed by the Newark Bay killifish. Previous DNA sequence analysis of the killifish CYP1A promoter revealed a 50 base pairs insertion that was present in some promoters but not in others. We studied the distribution of these inserts in the Newark Bay and Flax Pond (reference site) killifish populations using a PCR approach. Our results suggest that these insertions are not present in any of the Newark Bay killifish, but it occurred frequently on the promoters of Flax Pond killifish. Presentations at professional meetings: 1. 2002 Society of Environmental Toxicology and Chemistry 23rdAnnual Meeting. Sequence Comparison of the CYP1A promoter of AHR ligand resistant and responsive killifish (Fundulus heteroclitus). Arzuaga, X. and Elskus, A. 2. 2003 Pollutant Response in Marine Organisms 1ih International Symposium. Resistance to CYP1A induction in Newark Bay killifish (Fundulus heteroclitus) is not due to DNA methylation. Arzuaga, X., Calcano, W., and Elskus, A.
Effective start/end date9/26/029/25/04


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