Grants and Contracts Details
Description
Abstract
RNA interference discovered in nematode is being developed as an environmentally friendly and
target-specific pest control method. RNAi works well in coleopteran insects, and commercial
products targeting corn rootworm and Colorado potato beetle are registered in USA and other
countries. RNAi can be an effective alternative to the currently registered insecticides and has
the potential to integrate with natural enemies for effective eradication of coleopteran invasive
pests, such as the Asian longhorn beetle (ALB), the emerald ash borer and other invasive pests
that destroy trees. We have identified double-stranded RNA (dsRNA) targeting gene coding for
inhibitor of apoptosis (IAP) in ALB that triggers efficient RNAi and induces mortality. We have
developed methods to produce dsRNA in bacteria and showed their efficacy in killing ALB in
laboratory bioassays. We have also developed methods for producing large quantities of dsRNA
sufficient for field testing this product. This proposal requests funding for field testing of this
product to control ALB in the areas of South Carolina with current ALB infestation. Sufficient
quantities of dsIAP and dsGFP (control dsRNA) will be produced to inject six trees each three
times during next summer. The dsRNA produced will be tested by laboratory bioassays prior to
field testing. The dsIAP and dsGFP will be injected into trees selected based on their visual
ALB-infestation ratings conducted by state and federal regulatory personnel. ALB infestation
between treated and untreated trees will be compared. Six trees will be used as control trees and
injected with dsGFP. Six trees will receive dsIAP solution via trunk injection. Trees will be
treated first in late June when larvae are smallest and are still feeding on phloem. Two more
injections of dsRNA will be given in July and August to a total of three injections. Trees will be
destructively harvested in September to determine the extent of damage (galleries), and the
number of ALB larvae that survived the treatment. Successful completion of the proposed
research will provide methods to manage ALB in infested areas.
Status | Active |
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Effective start/end date | 9/30/24 → 9/29/25 |
Funding
- Animal and Plant Health Inspection Service: $135,039.00
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