Grants and Contracts Details
Description
Nicotine is the predominant alkaloid in tobacco (Nicotiana tobacum) and determines
smoke quality and causing addiction to smoking. Nicotine biosynthesis is regulated
by jasmonic acid (JA) signaling transduction pathway. MYC2, as a functionally conserved factor,
regulates downstream gene expressions directly or indirectly. Previously, our results
demonstrated that CrMYC2 interacts with transcriptional repressor CrGBF to antagonic inhibit
terpenoid indole alkaloid biosynthesis in Catharanthus roseus. Similarly, we also found that
overexpression of NtMYC2 also triggered the antagonic inhibition on nicotine biosynthesis gene
expression in tobacco. In this proposal, we will conduct transcriptomic analysis on NtMYC2
overexpression and RNAi transgenic lines and orthological comparison of other transcriptional
repressor sequence to investigate candidate transcription factor function as a the NtMYC2
midaited negative regulator in nicotine biosynthesis. Phylogenetic analysis, subcellular
localization, DNA-protein (or protein-protein) interaction assay, nicotine biosynthetic gene
expression levels and nicotine contents in transgenic overexpression and knock down plants
will be analyzed to verify the inhibition function of candidate regulator. The results will not only
further our understanding of the inhibitory regulation of nicotine biosynthesis, but also provide
gene resource which can be used in tobacco breeding program
Status | Finished |
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Effective start/end date | 11/1/19 → 10/31/22 |
Funding
- Yunnan Academy of Tobacco Agricultural Sciences: $89,978.00
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