Grants and Contracts Details
Description
The development of a real-time method for the intracellular measurement of RNA
production by optical imaging requires the development of a robust system. RNA
apatamers have been developed for imaging, but suffer the drawback of a weak signal in
the cellular mileau along with rapid photobleaching. We propose to develop aptamers
that avoid these deficiencies by designing specific fluorophores that will be emissive
upon binding an aptamer. This will be accomplished through the use of synthesis of
ligands coordinated around a ruthenium metal scaffold. These complexes will be
optimized for binding to the previously developed fluorescent apatamer, Spinach. In a
parallel effort the ruthenium compounds will be used to engineer a selective apatamer
using a random library combined with SELEX.
Status | Finished |
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Effective start/end date | 8/15/20 → 1/2/23 |
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