Investigation of Membrane Protein Folding through Manipulating the Rate of Protein Synthesis

Investigation of Membrane Protein Folding through Manipulating the Rate of Protein Synthesis. The long term goal is to elucidate folding pathways of membrane proteins. The objective of this proposal is to investigate the folding pathway of E. coli multidrug efflux pump, AcrB, through manipulating the rate of protein synthesis using synonymous mutations and ribosome stalling.