Grants and Contracts Details
Description
Plant pathogenic fungi secrete many proteins with diverse roles in plant-microbe
interactions. However, information on the temporal and spatial patterns of protein
secretion during fungal infection of plants is extremely limited, so in most cases it is not
clear which proteins are expressed/secreted at different infection stages. Likewise, it is
not known if secretion occurs at specific locations within invasive hyphae, and if secreted
proteins are targeted to specific regions of the pathogen-host interface. The goal of this
project is to gain a comprehensive insight into protein secretion by Magnaporthe oryzae
during infection of rice. We will accomplish this by creating GFP fusions to each of the
939 secreted proteins predicted in the genome sequence. Transformants carrying gene
fusions will be inoculated onto leaf sheaths and protein localization during penetration
and invasive growth will be determined by live-cell imaging of infection sites using
epifluorescence and confocal microscopy. The locations of selected proteins will be
validated by immunodetection with specific antibodies. This project will be facilitated
by the development of novel vectors for high throughput production of auto fluorescent
protein fusions in fungi, and by managing the workflow and data acquisition with a
Laboratory Information Management System designed for a similar project. The data
generated will be deposited in the MGOS database for access by the broader research
community. Knowledge gained in this project will further understanding in many areas
of plant microbe interactioris, which in turn will lead to the development of new ways to
protect the Nation's food supply.
Status | Finished |
---|---|
Effective start/end date | 2/1/08 → 1/31/12 |
Funding
- Cooperative State Research Education and Extension: $990,000.00
Fingerprint
Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.