Mitochondrial-Targeted Therapeutics for Treatment of Spinal Cord Injury

Successfully standardized methods for measurement of mitochondrial oxygen consumption employing the Seahorse Biosciences XF24 Flux Analyzer. With this method, only ~5ìg mitochondrial protein is needed to measure oxygen consumption (a 16-fold yield decrease in tissue needed). This breakthrough will allow us for the first the ability to assay total, synaptic and non-synaptic mitochondrial populations from the same animal. In addition to increased sensitivity, multiple samples can be analyzed at the same time.