Grants and Contracts Details
Description
Abstract
Overview of the project: This project will utilize fungi that are natural entomopathogens of the EAB
for delivery of lethal dsRNAi to the beetle. As fungal spores of entomopathogenic fungi may either
directly penetrate and parasitize the insect or be ingested, they provide an e icient vehicle to
deliver dsRNA directly to the insect. This project will test RNAi against several potential protein
targets in EAB for mortality against eggs, larvae, and adult EAB through ingestion or absorption of
dsRNA. The most e ective target gene(s) identi?ed will be expressed in the entomopathogenic
fungus, the registered biopesticide Beauveria bassiana strain GHA, and tested for increased
mortality and e icient RNAi with topical applications to EAB eggs and adults. Upon proving e icacy
of delivery through fungi, we will seek approval to deploy dsRNA and the fungi expressing dsRNA, in
a novel oviposition trap. The ?nal product will be a novel oviposition trap through which EAB adult
females will be infected with dsRNA or fungal spores and subsequently spread the fungus and RNAi
signal to eggs during oviposition and mating partners during mating.
Status | Active |
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Effective start/end date | 8/1/24 → 6/15/25 |
Funding
- Agricultural Research Service: $86,250.00
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