Role of Poly(ADP-ribose) Polymerase 1 in Regulating RNA polymerase II Elongation and mRNA Splicing

Subcontract abstract. PARP1 (poly-ADP-ribose polymerase), a chromatin-binding protein, is crucial for maintaining genomic integrity in mammals through regulation of proliferation, differentiation and aging. Although these roles are important, the mechanisms by which PARP1 interacts with DNA and proteins are poorly understood. We will contribute to defining those mechanisms through analysis of DNA and RNA binding equilibria and kinetics using data from electrophoretic mobility shift assays (EMSA), sedimentation equilibrium and velocity assays, and fluorescence assays. We will also contribute to experimental design for the further characterization of PARP1 interactions, to compilation of data for archive submission, and manuscript authorship and submission.