"Sample Prep" Sample Preparation for TSNA Analysis

A protocol is being compiled to deal with sample handling of cured tobacco for TSNA analysis. A draft protocol was based on the sample handling procedures of several tobacco companies, which included drying the tobacco at 77°F, 86°F or 95°F. The normal procedure in our laboratory is to air-dry the tobacco at ambient indoor temperature (about 68°F), because higher temperatures can affect TSNA levels. The objective of this study is to compare sample drying methods and to measure the effect of these drying methods on TSNAs. Treatments will include oven-drying at these three temperatures, air-drying at ambient indoor temperature, and freeze-drying. This work will give us a better understanding of the best way to prepare samples for TSNA analysis, in order to obtain reliable data. This is relevant for growers, because tobacco companies test growers’ tobacco for TSNAs, and may refuse to contract a grower if his TSNAs are too high. If the method of sample preparation is causing an increase in TSNAs, the grower might be unfairly penalized. It is also relevant for tobacco companies, because if the method of sample preparation affects TSNA measurement, the company will be operating with inaccurate data.