UK Spinal Cord and Brain Injury Research Center Core Grant

Grants and Contracts Details

Description

This is a competing renewal application of the existing National Institute of Neurological Disorders and Stroke (NINDS)-funded University of Kentucky (UK) Spinal Cord & Brain Injury Research Center (SCoBIRC) Core Grant (1P01 NS051220) that has enabled the creation of much needed core facilities within SCoBIRC which, over the past four years since their creation, have greatly accelerated and expanded the research capabilities of currently 15 SCoBIRC and other UK NIH-funded neuroscience faculty. who are qualifying principal investigators in that they are funded via one or more NINDS R01s, R21 or P01s. The SCoBIRC Core Facilities have been used to support funded NINDS and other NIH-funded (NIA, NIDA, neurologically-focused science projects and assist established and younger investigators in obtaining pilot data to support new NIH-funded neuroscience projects. The current application is directed at the maintenance, improvement, expansion of functional capabilities and equipment upgrading within the following five cores: " Administrative & Bioinformatics Core - the functions of this core will continue to include grants management and accounting, personnel record management, statistical analysis, shared secure data storage, computer support and poster printing; " Animal Modeling Core (formerly the Animal Model and SCI/TBI Models Core) - the functions of this core will continue to include the extensively utilized rodent surgical and in vivo neurotrauma modeling and physiological/elctrophysiological monitoring capabilities. However, this core will be expanded to include two new functions: viral vector construction and in vivo gene microinjection delivery and breeding and genotyping of genetically-modified rodents (transgenics and knockouts); " Behavioral Testing Core - the functions of this core are to provide a state of the art equipment, methodology for monitoring of cognitive, motor and sensory function in rodents subjected to experimental neurotrauma, ischemia or other genetic or neurological insults; " Microscopy Core (formerly Microscopy, Image Analysis and Stereology Core)- this heavily employed core will continue to provide a range of light and fluorescence microscopic, digital image analysis and stereological cell counting and tissue damage measurement capabilities. In addition, the core technical personnel will serve as a liaison with the shared UK Research Microscopy Center which houses transmission and scanning electron and multiple confocal microscopes. The core will also take on an expanded histological/immunohistochemical capability which will include training and method development; " Molecular Mechanisms Core (formerly, the Pharmacokinetics & Biomarker Core) - this core which has thus far been focused on gas chromatographic-mass spectrophotometric (GC-MS) and high performance liquid chromatographic analytical technologies for discovery of neurotrauma-related biomarkers and the pharmacokinetics of pharmacological test compounds will be expanded to include infra-red immunoblotting analysis, reverse transcriptase-polymerase chain reaction (RT-PCR) gene expression analysis and mitochondrial bioenergetic measurement capabilities Each core will continue to have a designated Core Director from among the SCoBIRC faculty and one or two dedicated full time technicians who are expert in the technologies included in each core. In the case of those cores which have the broader technical portfolio (e.g. Animal Modeling, Behavioral Testing, Microscopy, Molecular Mechanisms), an Assistant Director will be assigned to insure the needed technical coverage necessary for effective conduct of each technology intrinsic to the core. Moreover, part time technical staff from among the SCoBIRC laboratories are identified who are expert in the various methods. The Core Director, Assistant Director and/or the technical staff will be responsible for daily oversight, maintenance of core equipment, method development and improvement when necessary, training of other laboratory personnel and conduct of pilot studies for faculty and trainees (graduate students, postdoctoral fellows) who are interested in applying a particular technique to their research, but not yet sure that it will meet their particular needs. If the technique is found to be useful, the Core Directors and appropriate technicians will train designated technicians/trainees from that laboratory. An emphasis will be placed on using the cores to maximize the efficiency of established investigators and to promote the success of young investigators and trainees. The cores will continue to be housed in SCoBIRC assigned space on the ground and 4th floors of the Biological and Biomedical Sciences Research Building (BBSRB) which was completed in early 2005.
StatusFinished
Effective start/end date5/1/0511/30/15

Funding

  • National Institute of Neurological Disorders & Stroke: $3,227,910.00

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