Grants and Contracts Details
Description
The laboratory of Dr. Glenn Telling at the University of Kentucky will under subcontract
perfonnthe following tasks:
. Develop multiple iterations of transgenic mice expressing the nonnal cervid prion
protein (CerPrP).
. Detennine the susceptibility of Tg(CerPrP) mice to infection with the CWD prion
by: (1) observation for clinical disease, (2) necropsy, histopathology to map brain
lesions, (3) immunohistochemistry, ELISA and western blotting to demonstrate
PrPCWDin tissues, and (4) end-point in vivo titration.
. Use the most sensitive Tg(CerPrP) mouse strains to search for CWD infectivity in
body fluids and excreta from CWD+ deer by: (1) bioassay of blood and lymph
from deer in Tg(CerPrP) mice, and, (2) immunostaining of blood and lymphoid
cells in CWD-inoculated Tg(CerPrP) mice for PrPCWD.
. Evaluate the suitability of Tg(CerPrP) mice for typing of CWD strains by in vivo
inoculation of mice with CWD+ brain material from mule deer, white tail deer,
and elk followed by western blotting to assess PrPCWDglycofonns.
Status | Finished |
---|---|
Effective start/end date | 9/30/02 → 12/31/10 |
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