β-Amyloid deposition and tau phosphorylation in clinically characterized aged cats

E. Head; K. Moffat; P. Das; F. Sarsoza; W. W. Poon; G. Landsberg; Carl W. Cotman; M. P. Murphy

doi:10.1016/j.neurobiolaging.2004.06.015

β-Amyloid deposition and tau phosphorylation in clinically characterized aged cats

E. Head, K. Moffat, P. Das, F. Sarsoza, W. W. Poon, G. Landsberg, Carl W. Cotman, M. P. Murphy

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

The current study describes both Aβ and tau abnormalities that accumulate in the brains of aged (16-21 years), but not young (<4 years) clinically characterized cats. Diffuse plaques that were morphologically different from what is typically observed in the human brain could be detected with 4G8 (Aβ17-24) or an Aβ1-42-specific antibody but not with N-terminal Aβ or an Aβ1-40-specific antibody. SELDI-TOF mass spectrometry experiments indicated that cat brain Aβ consisted almost entirely of Aβ1-42. Markers of tau hyperphosphorylation (AT8 and PHF-1) labeled a subset of neurons in two aged animals. In the hilus of the hippocampus, a subset of AT8 positive neurons showed a sprouting morphology similar to that observed in human brain. Western blot analysis with antibodies against hyperphosphorylated tau indicated that tau is hyperphosphorylated in the aged cat and contains many of the same epitopes found in Alzheimer's disease (AD) brain. Thus, the aged cat brain develops AD-related lesions with important morphological and biochemical differences compared to human brain.

Original language	English
Pages (from-to)	749-763
Number of pages	15
Journal	Neurobiology of Aging
Volume	26
Issue number	5
DOIs	https://doi.org/10.1016/j.neurobiolaging.2004.06.015
State	Published - May 2005

Bibliographical note

Funding Information:
The authors would like to thank Miheala Nistor for her technical expertise. Funding provided in part by Pfizer Animal Health (K.M.), by the Mayo Foundation (M.P.M., P.D.), NIA P50 AG16573 (C.W.C.) and NIA AG12694 (C.W.C.).

Keywords

Alzheimer's disease
Amyloid β-peptide
Amyloid β-protein precursor
Down syndrome
Feline
Phosphorylated tau
Sprouting

ASJC Scopus subject areas

Clinical Neurology
Geriatrics and Gerontology
Aging
General Neuroscience
Developmental Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.neurobiolaging.2004.06.015

Cite this

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title = "β-Amyloid deposition and tau phosphorylation in clinically characterized aged cats",

abstract = "The current study describes both Aβ and tau abnormalities that accumulate in the brains of aged (16-21 years), but not young (<4 years) clinically characterized cats. Diffuse plaques that were morphologically different from what is typically observed in the human brain could be detected with 4G8 (Aβ17-24) or an Aβ1-42-specific antibody but not with N-terminal Aβ or an Aβ1-40-specific antibody. SELDI-TOF mass spectrometry experiments indicated that cat brain Aβ consisted almost entirely of Aβ1-42. Markers of tau hyperphosphorylation (AT8 and PHF-1) labeled a subset of neurons in two aged animals. In the hilus of the hippocampus, a subset of AT8 positive neurons showed a sprouting morphology similar to that observed in human brain. Western blot analysis with antibodies against hyperphosphorylated tau indicated that tau is hyperphosphorylated in the aged cat and contains many of the same epitopes found in Alzheimer's disease (AD) brain. Thus, the aged cat brain develops AD-related lesions with important morphological and biochemical differences compared to human brain.",

keywords = "Alzheimer's disease, Amyloid β-peptide, Amyloid β-protein precursor, Down syndrome, Feline, Phosphorylated tau, Sprouting",

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note = "Funding Information: The authors would like to thank Miheala Nistor for her technical expertise. Funding provided in part by Pfizer Animal Health (K.M.), by the Mayo Foundation (M.P.M., P.D.), NIA P50 AG16573 (C.W.C.) and NIA AG12694 (C.W.C.).",

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AU - Head, E.

AU - Moffat, K.

AU - Das, P.

AU - Sarsoza, F.

AU - Poon, W. W.

AU - Landsberg, G.

AU - Cotman, Carl W.

AU - Murphy, M. P.

N1 - Funding Information: The authors would like to thank Miheala Nistor for her technical expertise. Funding provided in part by Pfizer Animal Health (K.M.), by the Mayo Foundation (M.P.M., P.D.), NIA P50 AG16573 (C.W.C.) and NIA AG12694 (C.W.C.).

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N2 - The current study describes both Aβ and tau abnormalities that accumulate in the brains of aged (16-21 years), but not young (<4 years) clinically characterized cats. Diffuse plaques that were morphologically different from what is typically observed in the human brain could be detected with 4G8 (Aβ17-24) or an Aβ1-42-specific antibody but not with N-terminal Aβ or an Aβ1-40-specific antibody. SELDI-TOF mass spectrometry experiments indicated that cat brain Aβ consisted almost entirely of Aβ1-42. Markers of tau hyperphosphorylation (AT8 and PHF-1) labeled a subset of neurons in two aged animals. In the hilus of the hippocampus, a subset of AT8 positive neurons showed a sprouting morphology similar to that observed in human brain. Western blot analysis with antibodies against hyperphosphorylated tau indicated that tau is hyperphosphorylated in the aged cat and contains many of the same epitopes found in Alzheimer's disease (AD) brain. Thus, the aged cat brain develops AD-related lesions with important morphological and biochemical differences compared to human brain.

AB - The current study describes both Aβ and tau abnormalities that accumulate in the brains of aged (16-21 years), but not young (<4 years) clinically characterized cats. Diffuse plaques that were morphologically different from what is typically observed in the human brain could be detected with 4G8 (Aβ17-24) or an Aβ1-42-specific antibody but not with N-terminal Aβ or an Aβ1-40-specific antibody. SELDI-TOF mass spectrometry experiments indicated that cat brain Aβ consisted almost entirely of Aβ1-42. Markers of tau hyperphosphorylation (AT8 and PHF-1) labeled a subset of neurons in two aged animals. In the hilus of the hippocampus, a subset of AT8 positive neurons showed a sprouting morphology similar to that observed in human brain. Western blot analysis with antibodies against hyperphosphorylated tau indicated that tau is hyperphosphorylated in the aged cat and contains many of the same epitopes found in Alzheimer's disease (AD) brain. Thus, the aged cat brain develops AD-related lesions with important morphological and biochemical differences compared to human brain.

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β-Amyloid deposition and tau phosphorylation in clinically characterized aged cats

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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