β-Enolase is a marker of human myoblast heterogeneity prior to differentiation

Charlotte A. Peterson; Mildred Cho; Farzan Rastinejad; Helen M. Blau

doi:10.1016/0012-1606(92)90201-Q

β-Enolase is a marker of human myoblast heterogeneity prior to differentiation

Charlotte A. Peterson, Mildred Cho, Farzan Rastinejad, Helen M. Blau

Physical Therapy

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Abstract

In this report, we define a muscle-specific marker, β-enolase, that distinguishes proliferating myoblasts from different stages of development. Enolase exists as multiple isoforms and in the course of cardiac and skeletal muscle development the β isoform progressively replaces the α isoform. In skeletal muscle, this change in gene expression, unlike most developmental changes in myogenic gene expression, is evident in undifferentiated myoblasts. Whereas myoblasts from fetal tissues express α-enolase mRNA, β-enolase is the predominant mRNA expressed by myoblasts from postnatal tissues. Our results are consistent with the idea that distinct precursor myoblasts contribute to the diversity of fiber types characteristic of muscle tissue at different stages of development.

Original language	English
Pages (from-to)	626-629
Number of pages	4
Journal	Developmental Biology
Volume	151
Issue number	2
DOIs	https://doi.org/10.1016/0012-1606(92)90201-Q
State	Published - Jun 1992

Bibliographical note

Funding Information:
We thank Dr. Lydia Pan for providing the cDNA library and Dr. Simon Hughes for help with rat tissue dissections.F or the provision of plasmids, we thank Drs. Andrew Lassar and Harold Weintraub (MyoD), Hans Arnold (myf-5), Eric Olson (myogenin),J eff Miner and Barbara Wold (herculin/MRF4), Leslie Leinwand (myosin heavy chain), Nadia Rosenthal (myosin light chain), and Margaret Buckingham (cardiac actin). This work was supported by the California Affiliate of the AHA and the Katharine McCormick Fund for Women in Science( C.A.P.), NIH training grants in Pharmacology (M.C.) and in Neonatologya nd DevelopmentalB iology (M.C. and F.R.), a postdoctoral fellowship from the MDA (F.R.), and grants from the NIH, the NSF, the MDA, and the March of Dimes Birth Defect Foundation (H.M.B.).

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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title = "β-Enolase is a marker of human myoblast heterogeneity prior to differentiation",

abstract = "In this report, we define a muscle-specific marker, β-enolase, that distinguishes proliferating myoblasts from different stages of development. Enolase exists as multiple isoforms and in the course of cardiac and skeletal muscle development the β isoform progressively replaces the α isoform. In skeletal muscle, this change in gene expression, unlike most developmental changes in myogenic gene expression, is evident in undifferentiated myoblasts. Whereas myoblasts from fetal tissues express α-enolase mRNA, β-enolase is the predominant mRNA expressed by myoblasts from postnatal tissues. Our results are consistent with the idea that distinct precursor myoblasts contribute to the diversity of fiber types characteristic of muscle tissue at different stages of development.",

author = "Peterson, {Charlotte A.} and Mildred Cho and Farzan Rastinejad and Blau, {Helen M.}",

note = "Funding Information: We thank Dr. Lydia Pan for providing the cDNA library and Dr. Simon Hughes for help with rat tissue dissections.F or the provision of plasmids, we thank Drs. Andrew Lassar and Harold Weintraub (MyoD), Hans Arnold (myf-5), Eric Olson (myogenin),J eff Miner and Barbara Wold (herculin/MRF4), Leslie Leinwand (myosin heavy chain), Nadia Rosenthal (myosin light chain), and Margaret Buckingham (cardiac actin). This work was supported by the California Affiliate of the AHA and the Katharine McCormick Fund for Women in Science( C.A.P.), NIH training grants in Pharmacology (M.C.) and in Neonatologya nd DevelopmentalB iology (M.C. and F.R.), a postdoctoral fellowship from the MDA (F.R.), and grants from the NIH, the NSF, the MDA, and the March of Dimes Birth Defect Foundation (H.M.B.).",

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AU - Cho, Mildred

AU - Rastinejad, Farzan

AU - Blau, Helen M.

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AB - In this report, we define a muscle-specific marker, β-enolase, that distinguishes proliferating myoblasts from different stages of development. Enolase exists as multiple isoforms and in the course of cardiac and skeletal muscle development the β isoform progressively replaces the α isoform. In skeletal muscle, this change in gene expression, unlike most developmental changes in myogenic gene expression, is evident in undifferentiated myoblasts. Whereas myoblasts from fetal tissues express α-enolase mRNA, β-enolase is the predominant mRNA expressed by myoblasts from postnatal tissues. Our results are consistent with the idea that distinct precursor myoblasts contribute to the diversity of fiber types characteristic of muscle tissue at different stages of development.

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β-Enolase is a marker of human myoblast heterogeneity prior to differentiation

Abstract

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