褪黑素通过激活自噬抑制 MDA-MB-231 乳腺癌细胞的生长和转移

Translated title of the contribution: Melatonin inhibits growth and metastasis of MDA-MB-231 breast cancer cells by activating autophagy

Daoqiu Wu, Yi Zhang, Hongting Tang, Juan Yang, Mengxing Li, Honglin Liu, Qinshan Li

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Objective To investigate the effects of melatonin on the growth and metastasis of MDA-MB-231 breast cancer cells and explore the mechanism. Methods MDA-MB-231 cells were treated with 1, 3 or 5 mmol/L melatonin, and the changes in cell proliferation were examined using CCK-8 assay. Colony-forming assay and wound healing assay were used to assess the effects of melatonin treatmnent on colony-forming ability and migration of the cells. Flow cytometry and immunofluoresnce assay were employed to examine apoptosis and positive staining for autophagy-related proteins in the cells treated with 3 mmol/L melatonin. The effects of melatonin treatment alone or in combination with 3-methyladenine (3-MA) on the expressions of the proteins associated with autophagy (LC3, P62 and Beclin1), apoptosis (Bcl2 and Bax) and epithelial-mesenchymal transition (E-cadherin and Snail) were examined with Western blotting. Results Melatonin treatment significantly inhibited the proliferation of breast cancer cells in a concentration- and time-dependent manner (P<0.05), suppressed colony-forming ability and migration (P<0.01), and promoted apoptosis of the cells (P<0.01). Melatonin treatment alone significantly increased the expressions of Bax (P<0.05), E-cadherin, LC3-II/LC3-I, and Beclin1 and lowered the expressions of Bcl2 (P<0.05), Snail, P62 (P<0.05), and Bcl2/Bax ratio (P<0.01) in the cells, and caused enhanced positive staining of Beclin1 protein and attenuated staining of P62 protein. Compared with melatonin treatment alone, melatonin treatment combined with 3-MA significantly decreased the expressions of Beclin1 (P<0.001), LC3-II/LC3-I (P<0.05), Bax (P<0.01), and E-cadherin (P<0.001) and increased the expressions of Bcl2 (P<0.05), Snail, and Bcl2/Bax ratio (P<0.01). Conclusion Melatonin can induce autophagy of MDA-MB-231 breast cancer cells to inhibit cell proliferation and metastasis and promote cell apoptosis, and suppressing autophagy can weaken the inhibitory effect of melatonin on the growth and metastasis of breast cancer cells.

Translated title of the contributionMelatonin inhibits growth and metastasis of MDA-MB-231 breast cancer cells by activating autophagy
Original languageChinese (Simplified)
Pages (from-to)278-285
Number of pages8
JournalNan Fang Yi Ke Da Xue Xue Bao / Journal of Southern Medical University
Volume42
Issue number2
DOIs
StatePublished - 2022

Bibliographical note

Publisher Copyright:
© 2022 Editorial Department of Journal of Southern Medical University. All rights reserved.

Keywords

  • breast cancer
  • growth
  • melatonin
  • metastasis

ASJC Scopus subject areas

  • General Medicine

Fingerprint

Dive into the research topics of 'Melatonin inhibits growth and metastasis of MDA-MB-231 breast cancer cells by activating autophagy'. Together they form a unique fingerprint.

Cite this