褪黑素通过激活自噬抑制 MDA-MB-231 乳腺癌细胞的生长和转移

Daoqiu Wu; Yi Zhang; Hongting Tang; Juan Yang; Mengxing Li; Honglin Liu; Qinshan Li

doi:10.12122/j.issn.1673-4254.2022.02.16

褪黑素通过激活自噬抑制 MDA-MB-231 乳腺癌细胞的生长和转移

Translated title of the contribution: Melatonin inhibits growth and metastasis of MDA-MB-231 breast cancer cells by activating autophagy

Daoqiu Wu
, Yi Zhang
, Hongting Tang
, Juan Yang
, Mengxing Li
, Honglin Liu
, Qinshan Li

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Objective To investigate the effects of melatonin on the growth and metastasis of MDA-MB-231 breast cancer cells and explore the mechanism. Methods MDA-MB-231 cells were treated with 1, 3 or 5 mmol/L melatonin, and the changes in cell proliferation were examined using CCK-8 assay. Colony-forming assay and wound healing assay were used to assess the effects of melatonin treatmnent on colony-forming ability and migration of the cells. Flow cytometry and immunofluoresnce assay were employed to examine apoptosis and positive staining for autophagy-related proteins in the cells treated with 3 mmol/L melatonin. The effects of melatonin treatment alone or in combination with 3-methyladenine (3-MA) on the expressions of the proteins associated with autophagy (LC3, P62 and Beclin1), apoptosis (Bcl2 and Bax) and epithelial-mesenchymal transition (E-cadherin and Snail) were examined with Western blotting. Results Melatonin treatment significantly inhibited the proliferation of breast cancer cells in a concentration- and time-dependent manner (P<0.05), suppressed colony-forming ability and migration (P<0.01), and promoted apoptosis of the cells (P<0.01). Melatonin treatment alone significantly increased the expressions of Bax (P<0.05), E-cadherin, LC3-II/LC3-I, and Beclin1 and lowered the expressions of Bcl2 (P<0.05), Snail, P62 (P<0.05), and Bcl2/Bax ratio (P<0.01) in the cells, and caused enhanced positive staining of Beclin1 protein and attenuated staining of P62 protein. Compared with melatonin treatment alone, melatonin treatment combined with 3-MA significantly decreased the expressions of Beclin1 (P<0.001), LC3-II/LC3-I (P<0.05), Bax (P<0.01), and E-cadherin (P<0.001) and increased the expressions of Bcl2 (P<0.05), Snail, and Bcl2/Bax ratio (P<0.01). Conclusion Melatonin can induce autophagy of MDA-MB-231 breast cancer cells to inhibit cell proliferation and metastasis and promote cell apoptosis, and suppressing autophagy can weaken the inhibitory effect of melatonin on the growth and metastasis of breast cancer cells.

Translated title of the contribution	Melatonin inhibits growth and metastasis of MDA-MB-231 breast cancer cells by activating autophagy
Original language	Chinese (Simplified)
Pages (from-to)	278-285
Number of pages	8
Journal	Nan Fang Yi Ke Da Xue Xue Bao / Journal of Southern Medical University
Volume	42
Issue number	2
DOIs	https://doi.org/10.12122/j.issn.1673-4254.2022.02.16
State	Published - 2022

Bibliographical note

Publisher Copyright:
© 2022 Editorial Department of Journal of Southern Medical University. All rights reserved.

Funding

收稿日期：2021 -1 1-12 基金项目：国家自然科学基金（ 82173378 ， 81960476 ， 81460365 ， 81760039 ，81402451 ）；贵州省卫生健康委基金（ gzwkj 2021-160 ）；贵州省科技厅资助项目([2019]1270 ，[2020 ]4Y160)； Supported by National Natural Science Foundation of China (82173378,

Funders	Funder number
National Natural Science Foundation of P.R. China	4Y160, 81460365, 81760039, 2021-160, 82173378, 81402451, 81960476
National Natural Science Foundation of P.R. China

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

breast cancer
growth
melatonin
metastasis

ASJC Scopus subject areas

General Medicine

Access to Document

10.12122/j.issn.1673-4254.2022.02.16

Cite this

@article{ebea418701a44f7b8b11c02cb2eac255,

title = "褪黑素通过激活自噬抑制 MDA-MB-231 乳腺癌细胞的生长和转移",

abstract = "Objective To investigate the effects of melatonin on the growth and metastasis of MDA-MB-231 breast cancer cells and explore the mechanism. Methods MDA-MB-231 cells were treated with 1, 3 or 5 mmol/L melatonin, and the changes in cell proliferation were examined using CCK-8 assay. Colony-forming assay and wound healing assay were used to assess the effects of melatonin treatmnent on colony-forming ability and migration of the cells. Flow cytometry and immunofluoresnce assay were employed to examine apoptosis and positive staining for autophagy-related proteins in the cells treated with 3 mmol/L melatonin. The effects of melatonin treatment alone or in combination with 3-methyladenine (3-MA) on the expressions of the proteins associated with autophagy (LC3, P62 and Beclin1), apoptosis (Bcl2 and Bax) and epithelial-mesenchymal transition (E-cadherin and Snail) were examined with Western blotting. Results Melatonin treatment significantly inhibited the proliferation of breast cancer cells in a concentration- and time-dependent manner (P<0.05), suppressed colony-forming ability and migration (P<0.01), and promoted apoptosis of the cells (P<0.01). Melatonin treatment alone significantly increased the expressions of Bax (P<0.05), E-cadherin, LC3-II/LC3-I, and Beclin1 and lowered the expressions of Bcl2 (P<0.05), Snail, P62 (P<0.05), and Bcl2/Bax ratio (P<0.01) in the cells, and caused enhanced positive staining of Beclin1 protein and attenuated staining of P62 protein. Compared with melatonin treatment alone, melatonin treatment combined with 3-MA significantly decreased the expressions of Beclin1 (P<0.001), LC3-II/LC3-I (P<0.05), Bax (P<0.01), and E-cadherin (P<0.001) and increased the expressions of Bcl2 (P<0.05), Snail, and Bcl2/Bax ratio (P<0.01). Conclusion Melatonin can induce autophagy of MDA-MB-231 breast cancer cells to inhibit cell proliferation and metastasis and promote cell apoptosis, and suppressing autophagy can weaken the inhibitory effect of melatonin on the growth and metastasis of breast cancer cells.",

keywords = "breast cancer, growth, melatonin, metastasis",

author = "Daoqiu Wu and Yi Zhang and Hongting Tang and Juan Yang and Mengxing Li and Honglin Liu and Qinshan Li",

year = "2022",

doi = "10.12122/j.issn.1673-4254.2022.02.16",

language = "Chinese (Simplified)",

volume = "42",

pages = "278--285",

journal = "Nan Fang Yi Ke Da Xue Xue Bao / Journal of Southern Medical University",

issn = "1673-4254",

publisher = "Nanfang Yi Ke Da Xue Xue Bao Bian ji Bu",

number = "2",

}

TY - JOUR

T1 - 褪黑素通过激活自噬抑制 MDA-MB-231 乳腺癌细胞的生长和转移

AU - Wu, Daoqiu

AU - Zhang, Yi

AU - Tang, Hongting

AU - Yang, Juan

AU - Li, Mengxing

AU - Liu, Honglin

AU - Li, Qinshan

PY - 2022

Y1 - 2022

N2 - Objective To investigate the effects of melatonin on the growth and metastasis of MDA-MB-231 breast cancer cells and explore the mechanism. Methods MDA-MB-231 cells were treated with 1, 3 or 5 mmol/L melatonin, and the changes in cell proliferation were examined using CCK-8 assay. Colony-forming assay and wound healing assay were used to assess the effects of melatonin treatmnent on colony-forming ability and migration of the cells. Flow cytometry and immunofluoresnce assay were employed to examine apoptosis and positive staining for autophagy-related proteins in the cells treated with 3 mmol/L melatonin. The effects of melatonin treatment alone or in combination with 3-methyladenine (3-MA) on the expressions of the proteins associated with autophagy (LC3, P62 and Beclin1), apoptosis (Bcl2 and Bax) and epithelial-mesenchymal transition (E-cadherin and Snail) were examined with Western blotting. Results Melatonin treatment significantly inhibited the proliferation of breast cancer cells in a concentration- and time-dependent manner (P<0.05), suppressed colony-forming ability and migration (P<0.01), and promoted apoptosis of the cells (P<0.01). Melatonin treatment alone significantly increased the expressions of Bax (P<0.05), E-cadherin, LC3-II/LC3-I, and Beclin1 and lowered the expressions of Bcl2 (P<0.05), Snail, P62 (P<0.05), and Bcl2/Bax ratio (P<0.01) in the cells, and caused enhanced positive staining of Beclin1 protein and attenuated staining of P62 protein. Compared with melatonin treatment alone, melatonin treatment combined with 3-MA significantly decreased the expressions of Beclin1 (P<0.001), LC3-II/LC3-I (P<0.05), Bax (P<0.01), and E-cadherin (P<0.001) and increased the expressions of Bcl2 (P<0.05), Snail, and Bcl2/Bax ratio (P<0.01). Conclusion Melatonin can induce autophagy of MDA-MB-231 breast cancer cells to inhibit cell proliferation and metastasis and promote cell apoptosis, and suppressing autophagy can weaken the inhibitory effect of melatonin on the growth and metastasis of breast cancer cells.

AB - Objective To investigate the effects of melatonin on the growth and metastasis of MDA-MB-231 breast cancer cells and explore the mechanism. Methods MDA-MB-231 cells were treated with 1, 3 or 5 mmol/L melatonin, and the changes in cell proliferation were examined using CCK-8 assay. Colony-forming assay and wound healing assay were used to assess the effects of melatonin treatmnent on colony-forming ability and migration of the cells. Flow cytometry and immunofluoresnce assay were employed to examine apoptosis and positive staining for autophagy-related proteins in the cells treated with 3 mmol/L melatonin. The effects of melatonin treatment alone or in combination with 3-methyladenine (3-MA) on the expressions of the proteins associated with autophagy (LC3, P62 and Beclin1), apoptosis (Bcl2 and Bax) and epithelial-mesenchymal transition (E-cadherin and Snail) were examined with Western blotting. Results Melatonin treatment significantly inhibited the proliferation of breast cancer cells in a concentration- and time-dependent manner (P<0.05), suppressed colony-forming ability and migration (P<0.01), and promoted apoptosis of the cells (P<0.01). Melatonin treatment alone significantly increased the expressions of Bax (P<0.05), E-cadherin, LC3-II/LC3-I, and Beclin1 and lowered the expressions of Bcl2 (P<0.05), Snail, P62 (P<0.05), and Bcl2/Bax ratio (P<0.01) in the cells, and caused enhanced positive staining of Beclin1 protein and attenuated staining of P62 protein. Compared with melatonin treatment alone, melatonin treatment combined with 3-MA significantly decreased the expressions of Beclin1 (P<0.001), LC3-II/LC3-I (P<0.05), Bax (P<0.01), and E-cadherin (P<0.001) and increased the expressions of Bcl2 (P<0.05), Snail, and Bcl2/Bax ratio (P<0.01). Conclusion Melatonin can induce autophagy of MDA-MB-231 breast cancer cells to inhibit cell proliferation and metastasis and promote cell apoptosis, and suppressing autophagy can weaken the inhibitory effect of melatonin on the growth and metastasis of breast cancer cells.

KW - breast cancer

KW - growth

KW - melatonin

KW - metastasis

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UR - https://www.scopus.com/pages/publications/85127413695#tab=citedBy

U2 - 10.12122/j.issn.1673-4254.2022.02.16

DO - 10.12122/j.issn.1673-4254.2022.02.16

M3 - Article

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AN - SCOPUS:85127413695

SN - 1673-4254

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JO - Nan Fang Yi Ke Da Xue Xue Bao / Journal of Southern Medical University

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褪黑素通过激活自噬抑制 MDA-MB-231 乳腺癌细胞的生长和转移

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

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