1-14C linoleoyl-COA desaturation into diverse lipid classes of dracocephalum moldavica cotyledons

Mohammed Abdel-Reheem; David Hildebrand

1-¹⁴C linoleoyl-COA desaturation into diverse lipid classes of dracocephalum moldavica cotyledons

Mohammed Abdel-Reheem, David Hildebrand

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

To elucidate factors that contribute to the high 18:3 content of Dracocephalum moldavica (D. moldavica), a pulse-chase study of radiolabled ¹⁴C linoleoyl-CoA was carried out at an early and late stage of embryo triglyceride (TG) synthesis. In D. moldavica ¹⁴C-18:2 incorporation in PC increased and ¹⁴C-18:3 decreased, meanwhile the opposite was observed in TG (the incorporation of ¹⁴C-18:3 in TG is two-fold that of ¹⁴C-18:2 in PC). This suggests that both ω-3 desaturase is effectively desaturating ¹⁴C -18:2 in PC and acyltransferases are selectively transferring ¹⁴C -18:3 into TG. Also ¹⁴C -18:3 increased in D. moldavica TG indicates that most of the 18:2 was desaturated to 18:3 before transfer to the free fatty acid/acyl-CoA pool in D. moldavica.

Original language	English
Pages (from-to)	135-143
Number of pages	9
Journal	Life Science Journal
Volume	10
Issue number	SPL.ISSUE11
State	Published - 2013

Keywords

Dragonhead
Fatty acids
Lipid classes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology (all)

Cite this

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title = "1-14C linoleoyl-COA desaturation into diverse lipid classes of dracocephalum moldavica cotyledons",

abstract = "To elucidate factors that contribute to the high 18:3 content of Dracocephalum moldavica (D. moldavica), a pulse-chase study of radiolabled 14C linoleoyl-CoA was carried out at an early and late stage of embryo triglyceride (TG) synthesis. In D. moldavica 14C-18:2 incorporation in PC increased and 14C-18:3 decreased, meanwhile the opposite was observed in TG (the incorporation of 14C-18:3 in TG is two-fold that of 14C-18:2 in PC). This suggests that both ω-3 desaturase is effectively desaturating 14C -18:2 in PC and acyltransferases are selectively transferring 14C -18:3 into TG. Also 14C -18:3 increased in D. moldavica TG indicates that most of the 18:2 was desaturated to 18:3 before transfer to the free fatty acid/acyl-CoA pool in D. moldavica.",

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year = "2013",

language = "English",

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T1 - 1-14C linoleoyl-COA desaturation into diverse lipid classes of dracocephalum moldavica cotyledons

AU - Abdel-Reheem, Mohammed

AU - Hildebrand, David

PY - 2013

Y1 - 2013

N2 - To elucidate factors that contribute to the high 18:3 content of Dracocephalum moldavica (D. moldavica), a pulse-chase study of radiolabled 14C linoleoyl-CoA was carried out at an early and late stage of embryo triglyceride (TG) synthesis. In D. moldavica 14C-18:2 incorporation in PC increased and 14C-18:3 decreased, meanwhile the opposite was observed in TG (the incorporation of 14C-18:3 in TG is two-fold that of 14C-18:2 in PC). This suggests that both ω-3 desaturase is effectively desaturating 14C -18:2 in PC and acyltransferases are selectively transferring 14C -18:3 into TG. Also 14C -18:3 increased in D. moldavica TG indicates that most of the 18:2 was desaturated to 18:3 before transfer to the free fatty acid/acyl-CoA pool in D. moldavica.

AB - To elucidate factors that contribute to the high 18:3 content of Dracocephalum moldavica (D. moldavica), a pulse-chase study of radiolabled 14C linoleoyl-CoA was carried out at an early and late stage of embryo triglyceride (TG) synthesis. In D. moldavica 14C-18:2 incorporation in PC increased and 14C-18:3 decreased, meanwhile the opposite was observed in TG (the incorporation of 14C-18:3 in TG is two-fold that of 14C-18:2 in PC). This suggests that both ω-3 desaturase is effectively desaturating 14C -18:2 in PC and acyltransferases are selectively transferring 14C -18:3 into TG. Also 14C -18:3 increased in D. moldavica TG indicates that most of the 18:2 was desaturated to 18:3 before transfer to the free fatty acid/acyl-CoA pool in D. moldavica.

KW - Dragonhead

KW - Fatty acids

KW - Lipid classes

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M3 - Article

AN - SCOPUS:84886900166

SN - 1097-8135

VL - 10

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EP - 143

JO - Life Science Journal

JF - Life Science Journal

IS - SPL.ISSUE11

ER -

1-¹⁴C linoleoyl-COA desaturation into diverse lipid classes of dracocephalum moldavica cotyledons

Abstract

Keywords

ASJC Scopus subject areas

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