1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system

Hideaki Nagaoka; Yoshiyuki Mochida; Phimon Atsawasuwan; Masaru Kaku; Toshirou Kondoh; Mitsuo Yamauchi

doi:10.1016/j.bbrc.2008.10.036

1,25(OH)₂D₃ regulates collagen quality in an osteoblastic cell culture system

Hideaki Nagaoka, Yoshiyuki Mochida, Phimon Atsawasuwan, Masaru Kaku, Toshirou Kondoh, Mitsuo Yamauchi

Oral Health Science

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The active form of vitamin D, 1,25(OH)₂D₃, has a broad range of effects on bone, however, its role in the quality of bone matrix is not well understood. In this study, using an osteoblastic cell (MC3T3-E1) culture system, the effects of 1,25(OH)₂D₃ on collagen cross-linking and related enzymes, i.e., lysyl hydroxylases (LH1-3) and lysyl oxidases (LOX, LOXL1-4), were examined and compared to controls where cells were treated with cholecalciferol or ethanol. When compared to the controls, gene expressions of LH1, LH2b and LOXL2 were significantly upregulated by 1,25(OH)₂D₃ up to 72 h of culture. In addition, hydroxylysine (Hyl), Hyl aldehyde (Hyl^ald), Hyl^ald-derived cross-links and a total number of cross-links of collagen were significantly higher and the cross-link maturation was accelerated in the 1,25(OH)₂D₃ treated group. These results demonstrate that 1,25(OH)₂D₃ directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes.

Original language	English
Pages (from-to)	674-678
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	377
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2008.10.036
State	Published - Dec 12 2008

Bibliographical note

Funding Information:
This study was partially supported by NIH Grants NIH grant R01DE10489. The authors thank Drs. Ohta and Ishii at Teijin Pharma, Japan, for their valuable suggestions during the course of this study.

Keywords

Collagen cross-link
Collagen post-translational modification
MC3T3-E1
Vitamin D

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.10.036

Cite this

@article{a54864bfbd974aa9b41eb6ef5ea9e180,

title = "1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system",

abstract = "The active form of vitamin D, 1,25(OH)2D3, has a broad range of effects on bone, however, its role in the quality of bone matrix is not well understood. In this study, using an osteoblastic cell (MC3T3-E1) culture system, the effects of 1,25(OH)2D3 on collagen cross-linking and related enzymes, i.e., lysyl hydroxylases (LH1-3) and lysyl oxidases (LOX, LOXL1-4), were examined and compared to controls where cells were treated with cholecalciferol or ethanol. When compared to the controls, gene expressions of LH1, LH2b and LOXL2 were significantly upregulated by 1,25(OH)2D3 up to 72 h of culture. In addition, hydroxylysine (Hyl), Hyl aldehyde (Hylald), Hylald-derived cross-links and a total number of cross-links of collagen were significantly higher and the cross-link maturation was accelerated in the 1,25(OH)2D3 treated group. These results demonstrate that 1,25(OH)2D3 directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes.",

keywords = "Collagen cross-link, Collagen post-translational modification, MC3T3-E1, Vitamin D",

author = "Hideaki Nagaoka and Yoshiyuki Mochida and Phimon Atsawasuwan and Masaru Kaku and Toshirou Kondoh and Mitsuo Yamauchi",

note = "Funding Information: This study was partially supported by NIH Grants NIH grant R01DE10489. The authors thank Drs. Ohta and Ishii at Teijin Pharma, Japan, for their valuable suggestions during the course of this study.",

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T1 - 1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system

AU - Nagaoka, Hideaki

AU - Mochida, Yoshiyuki

AU - Atsawasuwan, Phimon

AU - Kaku, Masaru

AU - Kondoh, Toshirou

AU - Yamauchi, Mitsuo

N1 - Funding Information: This study was partially supported by NIH Grants NIH grant R01DE10489. The authors thank Drs. Ohta and Ishii at Teijin Pharma, Japan, for their valuable suggestions during the course of this study.

PY - 2008/12/12

Y1 - 2008/12/12

N2 - The active form of vitamin D, 1,25(OH)2D3, has a broad range of effects on bone, however, its role in the quality of bone matrix is not well understood. In this study, using an osteoblastic cell (MC3T3-E1) culture system, the effects of 1,25(OH)2D3 on collagen cross-linking and related enzymes, i.e., lysyl hydroxylases (LH1-3) and lysyl oxidases (LOX, LOXL1-4), were examined and compared to controls where cells were treated with cholecalciferol or ethanol. When compared to the controls, gene expressions of LH1, LH2b and LOXL2 were significantly upregulated by 1,25(OH)2D3 up to 72 h of culture. In addition, hydroxylysine (Hyl), Hyl aldehyde (Hylald), Hylald-derived cross-links and a total number of cross-links of collagen were significantly higher and the cross-link maturation was accelerated in the 1,25(OH)2D3 treated group. These results demonstrate that 1,25(OH)2D3 directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes.

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