2,2,6,6-Tetramethyl-4-amino [d17]piperidine-15n-l-oxyl as a spin label for cell surface carbohydrates.

Bennett T. Farmer; D. Allan Butterfield

doi:10.1080/00032718508066155

2,2,6,6-Tetramethyl-4-amino [d₁₇]piperidine-¹⁵n-l-oxyl as a spin label for cell surface carbohydrates.

Bennett T. Farmer, D. Allan Butterfield

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The first use of perdeuterated, ¹⁵N-Tempamine spin label to selectively monitor cell surface sialic acid of human erythrocyte membranes is reported. The molecular reasons for significantly increased sensitivity and significantly decreased scatter in the data over that of protonated, ¹⁴N-Tempamine are discussed. These advantages are illustrated by monitoring the effects of wheat-germ agglutinin on the motion of terminal sialic acid of membrane glycoconjugates.

Original language	English
Pages (from-to)	555-561
Number of pages	7
Journal	Analytical Letters
Volume	18
Issue number	5
DOIs	https://doi.org/10.1080/00032718508066155
State	Published - Jan 1985

Keywords

15N-spin labels
Perdeuterated
cell surface sialic acid
electron spin resonance
enhanced signal-to-noise ratio and increased precision

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

Access to Document

10.1080/00032718508066155

Cite this

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title = "2,2,6,6-Tetramethyl-4-amino [d17]piperidine-15n-l-oxyl as a spin label for cell surface carbohydrates.",

abstract = "The first use of perdeuterated, 15N-Tempamine spin label to selectively monitor cell surface sialic acid of human erythrocyte membranes is reported. The molecular reasons for significantly increased sensitivity and significantly decreased scatter in the data over that of protonated, 14N-Tempamine are discussed. These advantages are illustrated by monitoring the effects of wheat-germ agglutinin on the motion of terminal sialic acid of membrane glycoconjugates.",

keywords = "15N-spin labels, Perdeuterated, cell surface sialic acid, electron spin resonance, enhanced signal-to-noise ratio and increased precision",

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T1 - 2,2,6,6-Tetramethyl-4-amino [d17]piperidine-15n-l-oxyl as a spin label for cell surface carbohydrates.

AU - Farmer, Bennett T.

AU - Allan Butterfield, D.

PY - 1985/1

Y1 - 1985/1

N2 - The first use of perdeuterated, 15N-Tempamine spin label to selectively monitor cell surface sialic acid of human erythrocyte membranes is reported. The molecular reasons for significantly increased sensitivity and significantly decreased scatter in the data over that of protonated, 14N-Tempamine are discussed. These advantages are illustrated by monitoring the effects of wheat-germ agglutinin on the motion of terminal sialic acid of membrane glycoconjugates.

AB - The first use of perdeuterated, 15N-Tempamine spin label to selectively monitor cell surface sialic acid of human erythrocyte membranes is reported. The molecular reasons for significantly increased sensitivity and significantly decreased scatter in the data over that of protonated, 14N-Tempamine are discussed. These advantages are illustrated by monitoring the effects of wheat-germ agglutinin on the motion of terminal sialic acid of membrane glycoconjugates.

KW - 15N-spin labels

KW - Perdeuterated

KW - cell surface sialic acid

KW - electron spin resonance

KW - enhanced signal-to-noise ratio and increased precision

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JO - Analytical Letters

JF - Analytical Letters

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