Abstract
The antioxidant properties of 5-aminosalicylic acid in vitro were evaluated in a synaptosomal membrane system prepared from gerbil cortical synaptosomes using EPR spin labeling and spectroscopic techniques. MAL-6 (2,2,6,6-tetramethyl-4-maleimidopiperidin-1-oxyl) and 5-NS (5-nitroxide stearate) spin labels were used to assess changes in protein oxidation and membrane lipid fluidity, respectively. Synaptosomal membranes were subjected to oxidative stress by incubation with 1 mM azo-bis(isobutyronitrile) (AIBN) or 1 mM 2,2′-azobis(amidino propane) dihydrochloride (AAPH) at 37°C for 30 minutes. The EPR analyses of the samples showed significant oxidation of synaptosomal proteins and a decrease in membrane fluidity. 5-Aminosalicylic acid also was evaluated by means of FRAP (the ferric reducing ability of plasma) test as a potential antioxidant. 5-Aminosalicylic acid also showed protection against the oxidation in gerbil cortical synaptosomes system caused by AIBN and AAPH. These results are consistent with the notion of antioxidant protection against free radical induced oxidative stress in synaptosomal membrane system by this agent.
| Original language | English |
|---|---|
| Pages (from-to) | 23-29 |
| Number of pages | 7 |
| Journal | Neurochemical Research |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2001 |
Bibliographical note
Funding Information:This work was supported in part by NIH grants (AG-05119; AG-10836; AG-12423).
Keywords
- (5-ASA)
- (AAPH)
- (AIBN)
- 2,2′-azobis(amino propane) dihydrochloride
- 5-aminosalicylic acid
- Antioxidants
- Azo-bis(isobutyronitrile)
- EPR
- Reactive oxygen species
- Synaptosomal membranes
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience