5-Hydroxytryptophan strongly stimulates serotonin synthesis in Holstein steers

E. E.L. Valente, J. L. Klotz, D. L. Harmon

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Although serotonin has been extensively studied in many species, there is a lack of information in ruminants, and no research has been evaluated if its precursor, 5-hydroxytryptophan (5-HTP), administered into the abomasum may be used as a means to manipulate serotonin metabolism. Thus, the objective of this study was to evaluate if intra-abomasal infusion of 5-HTP increases circulating serotonin in the steer. Eight Holstein steers (471 ± 8.9 kg) were used in a replicated 4 × 4 Latin Square design experiment. The treatments were intra-abomasal infusion of 5-HTP at 0.5, 1, 2.5, and 5 mg/kg BW. Blood was collected at 0, 2, 4, 6, 8, and 24 h after infusion. The serum concentration of 5-HTP increased quadratically (P = 0.005) with a peak at 2 h after administration. The 5-HTP administration increased (P < 0.05) serum serotonin in comparison with baseline with no difference (P > 0.05) between the doses of 5-HTP. When 5-HTP was dosed at 2.5 mg/kg BW or higher, intake decreased, and there was an altered manure consistency. The serum 5-hydroxyindole acetic acid concentrations followed the same pattern as 5-HTP. Plasma glucose content was not affected (P > 0.05) by 5-HTP dosing. However, free fatty acids concentration in the plasma was lower (P > 0.05) compared with baseline for the infusion levels of 0.5 and 1 mg/kg BW. Intra-abomasal infusion of 5-HTP efficiently increases serum serotonin cattle.

Original languageEnglish
Article number106560
JournalDomestic Animal Endocrinology
Volume74
DOIs
StatePublished - Jan 2021

Bibliographical note

Funding Information:
The authors would like to acknowledge the support of K. Vanzant, B. Cotton and W. Lin in the conduct of this research. This work is funded by Hatch Capacity Grant Project no. KY007088 from the USDA National Institute of Food and Agriculture and Project 201807121511 from USDA/ARS and the University of Kentucky Agricultural Experiment Station.

Funding Information:
The authors would like to acknowledge the support of K. Vanzant, B. Cotton and W. Lin in the conduct of this research. This work is funded by Hatch Capacity Grant Project no. KY007088 from the USDA National Institute of Food and Agriculture and Project 201807121511 from USDA/ARS and the University of Kentucky Agricultural Experiment Station .

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

  • 5-HT
  • 5-HTP
  • Cattle
  • Neurotransmitter

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

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