TY - JOUR
T1 - 8-Hydroxy-2′-deoxyguanosine Formation and DNA Damage Induced by Sulfur Trioxide Anion Radicals
AU - Shi, X. L.
AU - Mao, Y.
PY - 1994/11/30
Y1 - 1994/11/30
N2 - The 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation and DNA damage by sulfur trioxide anion radicals (SO·-3) were investigated using ESR spin trapping, HPLC, and electrophoretic assays. Sulfite (SO2-3) autoxidation generated both hydroxyl (·OH) and SO·-3 radicals. Oxidation of SO2-3 by chromium (VI) generated only SO·-3 with much enhanced yield. Incubation of 2′-deoxyguanosine (dG) with SO2-3 generated 8-OHdG albeit at low yield. Chromium (VI) enhanced the yield four-fold. Electrophoretic assays showed that SO·-3 radicals generated by chromium (VI) oxidation of SO2-3 caused DNA double strand breaks. The results demonstrate that SO·-3 radicals are capable of causing dG hydroxylation and DNA double strand breaks.
AB - The 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation and DNA damage by sulfur trioxide anion radicals (SO·-3) were investigated using ESR spin trapping, HPLC, and electrophoretic assays. Sulfite (SO2-3) autoxidation generated both hydroxyl (·OH) and SO·-3 radicals. Oxidation of SO2-3 by chromium (VI) generated only SO·-3 with much enhanced yield. Incubation of 2′-deoxyguanosine (dG) with SO2-3 generated 8-OHdG albeit at low yield. Chromium (VI) enhanced the yield four-fold. Electrophoretic assays showed that SO·-3 radicals generated by chromium (VI) oxidation of SO2-3 caused DNA double strand breaks. The results demonstrate that SO·-3 radicals are capable of causing dG hydroxylation and DNA double strand breaks.
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U2 - 10.1006/bbrc.1994.2641
DO - 10.1006/bbrc.1994.2641
M3 - Article
C2 - 7999014
AN - SCOPUS:0028130656
SN - 0006-291X
VL - 205
SP - 141
EP - 147
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -