8-Hydroxy-2′-deoxyguanosine Formation and DNA Damage Induced by Sulfur Trioxide Anion Radicals

X. L. Shi, Y. Mao

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

The 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation and DNA damage by sulfur trioxide anion radicals (SO·-3) were investigated using ESR spin trapping, HPLC, and electrophoretic assays. Sulfite (SO2-3) autoxidation generated both hydroxyl (·OH) and SO·-3 radicals. Oxidation of SO2-3 by chromium (VI) generated only SO·-3 with much enhanced yield. Incubation of 2′-deoxyguanosine (dG) with SO2-3 generated 8-OHdG albeit at low yield. Chromium (VI) enhanced the yield four-fold. Electrophoretic assays showed that SO·-3 radicals generated by chromium (VI) oxidation of SO2-3 caused DNA double strand breaks. The results demonstrate that SO·-3 radicals are capable of causing dG hydroxylation and DNA double strand breaks.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume205
Issue number1
DOIs
StatePublished - Nov 30 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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