8-Hydroxy-2′-deoxyguanosine Formation and DNA Damage Induced by Sulfur Trioxide Anion Radicals

X. L. Shi; Y. Mao

doi:10.1006/bbrc.1994.2641

8-Hydroxy-2′-deoxyguanosine Formation and DNA Damage Induced by Sulfur Trioxide Anion Radicals

X. L. Shi, Y. Mao

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Abstract

The 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation and DNA damage by sulfur trioxide anion radicals (SO^·-₃) were investigated using ESR spin trapping, HPLC, and electrophoretic assays. Sulfite (SO^2-₃) autoxidation generated both hydroxyl (^·OH) and SO^·-₃ radicals. Oxidation of SO^2-₃ by chromium (VI) generated only SO^·-₃ with much enhanced yield. Incubation of 2′-deoxyguanosine (dG) with SO^2-₃ generated 8-OHdG albeit at low yield. Chromium (VI) enhanced the yield four-fold. Electrophoretic assays showed that SO^·-₃ radicals generated by chromium (VI) oxidation of SO^2-₃ caused DNA double strand breaks. The results demonstrate that SO^·-₃ radicals are capable of causing dG hydroxylation and DNA double strand breaks.

Original language	English
Pages (from-to)	141-147
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	205
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1994.2641
State	Published - Nov 30 1994

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "The 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation and DNA damage by sulfur trioxide anion radicals (SO·-3) were investigated using ESR spin trapping, HPLC, and electrophoretic assays. Sulfite (SO2-3) autoxidation generated both hydroxyl (·OH) and SO·-3 radicals. Oxidation of SO2-3 by chromium (VI) generated only SO·-3 with much enhanced yield. Incubation of 2′-deoxyguanosine (dG) with SO2-3 generated 8-OHdG albeit at low yield. Chromium (VI) enhanced the yield four-fold. Electrophoretic assays showed that SO·-3 radicals generated by chromium (VI) oxidation of SO2-3 caused DNA double strand breaks. The results demonstrate that SO·-3 radicals are capable of causing dG hydroxylation and DNA double strand breaks.",

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N2 - The 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation and DNA damage by sulfur trioxide anion radicals (SO·-3) were investigated using ESR spin trapping, HPLC, and electrophoretic assays. Sulfite (SO2-3) autoxidation generated both hydroxyl (·OH) and SO·-3 radicals. Oxidation of SO2-3 by chromium (VI) generated only SO·-3 with much enhanced yield. Incubation of 2′-deoxyguanosine (dG) with SO2-3 generated 8-OHdG albeit at low yield. Chromium (VI) enhanced the yield four-fold. Electrophoretic assays showed that SO·-3 radicals generated by chromium (VI) oxidation of SO2-3 caused DNA double strand breaks. The results demonstrate that SO·-3 radicals are capable of causing dG hydroxylation and DNA double strand breaks.

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8-Hydroxy-2′-deoxyguanosine Formation and DNA Damage Induced by Sulfur Trioxide Anion Radicals

Abstract

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