The 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation and DNA damage by sulfur trioxide anion radicals (SO·-3) were investigated using ESR spin trapping, HPLC, and electrophoretic assays. Sulfite (SO2-3) autoxidation generated both hydroxyl (·OH) and SO·-3 radicals. Oxidation of SO2-3 by chromium (VI) generated only SO·-3 with much enhanced yield. Incubation of 2′-deoxyguanosine (dG) with SO2-3 generated 8-OHdG albeit at low yield. Chromium (VI) enhanced the yield four-fold. Electrophoretic assays showed that SO·-3 radicals generated by chromium (VI) oxidation of SO2-3 caused DNA double strand breaks. The results demonstrate that SO·-3 radicals are capable of causing dG hydroxylation and DNA double strand breaks.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Nov 30 1994|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology