[8] Micromethod for the Measurement of Acetyl Phosphate and Acetyl Coenzyme A

Arthur G. Hunt

doi:10.1016/0076-6879(86)22146-1

[8] Micromethod for the Measurement of Acetyl Phosphate and Acetyl Coenzyme A

Arthur G. Hunt

Plant and Soil Sciences

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Abstract

The method described here utilizes two enzymes involved with acetyl-CoA and acetyl phosphate metabolism in bacteria to measure these compounds. Basically, the acetyl-CoA and acetyl phosphate in perchloric acid extracts are converted to ATP, in the presence of [³H]ADP of high specific activity, using the enzymes phosphotransacetylase (PTA) and acetate kinase (AK).

Original language	English
Pages (from-to)	43-50
Number of pages	8
Journal	Methods in Enzymology
Volume	122
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(86)22146-1
State	Published - Jan 1 1986

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/0076-6879(86)22146-1

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title = "[8] Micromethod for the Measurement of Acetyl Phosphate and Acetyl Coenzyme A",

abstract = "The method described here utilizes two enzymes involved with acetyl-CoA and acetyl phosphate metabolism in bacteria to measure these compounds. Basically, the acetyl-CoA and acetyl phosphate in perchloric acid extracts are converted to ATP, in the presence of [3H]ADP of high specific activity, using the enzymes phosphotransacetylase (PTA) and acetate kinase (AK).",

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AB - The method described here utilizes two enzymes involved with acetyl-CoA and acetyl phosphate metabolism in bacteria to measure these compounds. Basically, the acetyl-CoA and acetyl phosphate in perchloric acid extracts are converted to ATP, in the presence of [3H]ADP of high specific activity, using the enzymes phosphotransacetylase (PTA) and acetate kinase (AK).

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[8] Micromethod for the Measurement of Acetyl Phosphate and Acetyl Coenzyme A

Abstract

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