TY - JOUR
T1 - [8] Micromethod for the Measurement of Acetyl Phosphate and Acetyl Coenzyme A
AU - Hunt, Arthur G.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - The method described here utilizes two enzymes involved with acetyl-CoA and acetyl phosphate metabolism in bacteria to measure these compounds. Basically, the acetyl-CoA and acetyl phosphate in perchloric acid extracts are converted to ATP, in the presence of [3H]ADP of high specific activity, using the enzymes phosphotransacetylase (PTA) and acetate kinase (AK).
AB - The method described here utilizes two enzymes involved with acetyl-CoA and acetyl phosphate metabolism in bacteria to measure these compounds. Basically, the acetyl-CoA and acetyl phosphate in perchloric acid extracts are converted to ATP, in the presence of [3H]ADP of high specific activity, using the enzymes phosphotransacetylase (PTA) and acetate kinase (AK).
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U2 - 10.1016/0076-6879(86)22146-1
DO - 10.1016/0076-6879(86)22146-1
M3 - Article
C2 - 3517571
AN - SCOPUS:0022559097
SN - 0076-6879
VL - 122
SP - 43
EP - 50
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -