[8] Micromethod for the Measurement of Acetyl Phosphate and Acetyl Coenzyme A

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6 Scopus citations

Abstract

The method described here utilizes two enzymes involved with acetyl-CoA and acetyl phosphate metabolism in bacteria to measure these compounds. Basically, the acetyl-CoA and acetyl phosphate in perchloric acid extracts are converted to ATP, in the presence of [3H]ADP of high specific activity, using the enzymes phosphotransacetylase (PTA) and acetate kinase (AK).

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalMethods in Enzymology
Volume122
Issue numberC
DOIs
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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