A routine procedure in the study of polyadenylation involves the determinations of the junctions of the mRNA body and the poly(A) tail (the poly(A) site). This is typically accomplished by selectively amplifying the 3'-ends of cDNAs (3'-Rapid Amplification of cDNA Ends, or 3'-RACE), followed by sequencing of individual clones. We have developed a modification of the standard 3'-RACE protocol that couples high specificity with the ability to characterize thousands (or more) of individual sequences. This protocol may be used for numerous purposes, including the confirmation of results obtained using high throughput sequencing technologies.
|Title of host publication||Polyadenylation in Plants|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||10|
|State||Published - Dec 8 2014|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media New York 2015. All rights are reserved.
- 3' RACE protocol
- Ion torrent
- Polyadenylation sites
ASJC Scopus subject areas
- Agricultural and Biological Sciences (all)
- Medicine (all)
- Biochemistry, Genetics and Molecular Biology (all)