A 3' RACE protocol to confirm polyadenylation sites

Liuyin Ma; Arthur G. Hunt

doi:10.1007/978-1-4939-2175-1_12

A 3' RACE protocol to confirm polyadenylation sites

Liuyin Ma, Arthur G. Hunt

Plant and Soil Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

A routine procedure in the study of polyadenylation involves the determinations of the junctions of the mRNA body and the poly(A) tail (the poly(A) site). This is typically accomplished by selectively amplifying the 3'-ends of cDNAs (3'-Rapid Amplification of cDNA Ends, or 3'-RACE), followed by sequencing of individual clones. We have developed a modification of the standard 3'-RACE protocol that couples high specificity with the ability to characterize thousands (or more) of individual sequences. This protocol may be used for numerous purposes, including the confirmation of results obtained using high throughput sequencing technologies.

Original language	English
Title of host publication	Polyadenylation in Plants
Subtitle of host publication	Methods and Protocols
Pages	135-144
Number of pages	10
ISBN (Electronic)	9781493921751
DOIs	https://doi.org/10.1007/978-1-4939-2175-1_12
State	Published - Dec 8 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015. All rights are reserved.

Keywords

3' RACE protocol
Ion torrent
MiSeq
Polyadenylation sites

ASJC Scopus subject areas

General Agricultural and Biological Sciences
General Medicine
General Biochemistry, Genetics and Molecular Biology

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10.1007/978-1-4939-2175-1_12

Cite this

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A 3' RACE protocol to confirm polyadenylation sites

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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