A 3′ race protocol to confirm polyadenylation sites

Liuyin Ma; Arthur G. Hunt

doi:10.1007/978-1-4939-2175-1_12

A 3′ race protocol to confirm polyadenylation sites

Liuyin Ma, Arthur G. Hunt

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A routine procedure in the study of polyadenylation involves the determinations of the junctions of the mRNA body and the poly(A) tail (the poly(A) site). This is typically accomplished by selectively amplifying the 3′-ends of cDNAs (3′-Rapid Amplifi cation of cDNA Ends, or 3′-RACE), followed by sequencing of individual clones. We have developed a modifi cation of the standard 3′-RACE protocol that couples high specifi city with the ability to characterize thousands (or more) of individual sequences. This protocol may be used for numerous purposes, including the confi rmation of results obtained using high throughput sequencing technologies.

Original language	English
Pages (from-to)	135-144
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1255
DOIs	https://doi.org/10.1007/978-1-4939-2175-1_12
State	Published - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

3′ RACE protocol
Ion torrent
MiSeq
Polyadenylation sites

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2175-1_12

Cite this

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title = "A 3′ race protocol to confirm polyadenylation sites",

abstract = "A routine procedure in the study of polyadenylation involves the determinations of the junctions of the mRNA body and the poly(A) tail (the poly(A) site). This is typically accomplished by selectively amplifying the 3′-ends of cDNAs (3′-Rapid Amplifi cation of cDNA Ends, or 3′-RACE), followed by sequencing of individual clones. We have developed a modifi cation of the standard 3′-RACE protocol that couples high specifi city with the ability to characterize thousands (or more) of individual sequences. This protocol may be used for numerous purposes, including the confi rmation of results obtained using high throughput sequencing technologies.",

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N2 - A routine procedure in the study of polyadenylation involves the determinations of the junctions of the mRNA body and the poly(A) tail (the poly(A) site). This is typically accomplished by selectively amplifying the 3′-ends of cDNAs (3′-Rapid Amplifi cation of cDNA Ends, or 3′-RACE), followed by sequencing of individual clones. We have developed a modifi cation of the standard 3′-RACE protocol that couples high specifi city with the ability to characterize thousands (or more) of individual sequences. This protocol may be used for numerous purposes, including the confi rmation of results obtained using high throughput sequencing technologies.

AB - A routine procedure in the study of polyadenylation involves the determinations of the junctions of the mRNA body and the poly(A) tail (the poly(A) site). This is typically accomplished by selectively amplifying the 3′-ends of cDNAs (3′-Rapid Amplifi cation of cDNA Ends, or 3′-RACE), followed by sequencing of individual clones. We have developed a modifi cation of the standard 3′-RACE protocol that couples high specifi city with the ability to characterize thousands (or more) of individual sequences. This protocol may be used for numerous purposes, including the confi rmation of results obtained using high throughput sequencing technologies.

KW - 3′ RACE protocol

KW - Ion torrent

KW - MiSeq

KW - Polyadenylation sites

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DO - 10.1007/978-1-4939-2175-1_12

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AN - SCOPUS:84954594934

SN - 1064-3745

VL - 1255

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

A 3′ race protocol to confirm polyadenylation sites

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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