A 3′ race protocol to confirm polyadenylation sites

Liuyin Ma, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


A routine procedure in the study of polyadenylation involves the determinations of the junctions of the mRNA body and the poly(A) tail (the poly(A) site). This is typically accomplished by selectively amplifying the 3′-ends of cDNAs (3′-Rapid Amplifi cation of cDNA Ends, or 3′-RACE), followed by sequencing of individual clones. We have developed a modifi cation of the standard 3′-RACE protocol that couples high specifi city with the ability to characterize thousands (or more) of individual sequences. This protocol may be used for numerous purposes, including the confi rmation of results obtained using high throughput sequencing technologies.

Original languageEnglish
Pages (from-to)135-144
Number of pages10
JournalMethods in Molecular Biology
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.


  • 3′ RACE protocol
  • Ion torrent
  • MiSeq
  • Polyadenylation sites

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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