A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths

Tiago R. Correia; Bernardo P. Antunes; Pedro H. Castilho; José C. Nunes; Maria T. Pessoa De Amorim; Isabel C. Escobar; João A. Queiroz; Ilídio J. Correia; António M. Morão

doi:10.1016/j.seppur.2013.03.049

A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths

Tiago R. Correia, Bernardo P. Antunes, Pedro H. Castilho, José C. Nunes, Maria T. Pessoa De Amorim, Isabel C. Escobar, João A. Queiroz, Ilídio J. Correia, António M. Morão

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.

Original language	English
Pages (from-to)	20-25
Number of pages	6
Journal	Separation and Purification Technology
Volume	112
DOIs	https://doi.org/10.1016/j.seppur.2013.03.049
State	Published - 2013

Bibliographical note

Funding Information:
This work was supported by the Portuguese Foundation for Science and Technology (FCT) , ( PTDC/EME-TME/103375/2008 and PTDC/EBB-BIO/114320/2009 ). To Ricardo Fradique for helping in the production of the graphical abstract.

Funding

This work was supported by the Portuguese Foundation for Science and Technology (FCT) , ( PTDC/EME-TME/103375/2008 and PTDC/EBB-BIO/114320/2009 ). To Ricardo Fradique for helping in the production of the graphical abstract.

Funders	Funder number
Fundação para a Ciência e Tecnologia I.P.	PTDC/EME-TME/103375/2008, PTDC/EBB-BIO/114320/2009

Keywords

Bi-layer membrane
DNA
Electrospinning
Lysate
Microfiltration
Plasmid

ASJC Scopus subject areas

Analytical Chemistry
Filtration and Separation

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10.1016/j.seppur.2013.03.049

Cite this

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title = "A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths",

abstract = "The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.",

keywords = "Bi-layer membrane, DNA, Electrospinning, Lysate, Microfiltration, Plasmid",

author = "Correia, {Tiago R.} and Antunes, {Bernardo P.} and Castilho, {Pedro H.} and Nunes, {Jos{\'e} C.} and {Pessoa De Amorim}, {Maria T.} and Escobar, {Isabel C.} and Queiroz, {Jo{\~a}o A.} and Correia, {Il{\'i}dio J.} and Mor{\~a}o, {Ant{\'o}nio M.}",

note = "Funding Information: This work was supported by the Portuguese Foundation for Science and Technology (FCT) , ( PTDC/EME-TME/103375/2008 and PTDC/EBB-BIO/114320/2009 ). To Ricardo Fradique for helping in the production of the graphical abstract.",

year = "2013",

doi = "10.1016/j.seppur.2013.03.049",

language = "English",

volume = "112",

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AU - Correia, Tiago R.

AU - Antunes, Bernardo P.

AU - Castilho, Pedro H.

AU - Nunes, José C.

AU - Pessoa De Amorim, Maria T.

AU - Escobar, Isabel C.

AU - Queiroz, João A.

AU - Correia, Ilídio J.

AU - Morão, António M.

N1 - Funding Information: This work was supported by the Portuguese Foundation for Science and Technology (FCT) , ( PTDC/EME-TME/103375/2008 and PTDC/EBB-BIO/114320/2009 ). To Ricardo Fradique for helping in the production of the graphical abstract.

PY - 2013

Y1 - 2013

N2 - The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.

AB - The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.

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KW - Microfiltration

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A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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