TY - JOUR
T1 - A bioinformatics approach to identifying tail-anchored proteins in the human genome
AU - Kalbfleisch, Ted
AU - Cambon, Alex
AU - Wattenberg, Binks W.
PY - 2007/12
Y1 - 2007/12
N2 - Intracellular proteins with a carboxy-terminal transmembrane domain and the amino-terminus oriented toward the cytosol are known as 'tail-anchored' proteins. Tail-anchored proteins have been of considerable interest because several important classes of proteins, including the vesicle-targeting/fusion proteins known as SNAREs and the apoptosis-related proteins of the Bcl-2 family, among others, utilize this unique membrane-anchoring motif. Here, we use a bioinformatic technique to develop a comprehensive list of potentially tail-anchored proteins in the human genome. Our final list contains 411 entries derived from 325 unique genes. We also analyzed both known and predicted tail-anchored proteins with respect to the amino acid composition of the transmembrane segments. This analysis revealed a distinctive composition of the membrane anchor in SNARE proteins.
AB - Intracellular proteins with a carboxy-terminal transmembrane domain and the amino-terminus oriented toward the cytosol are known as 'tail-anchored' proteins. Tail-anchored proteins have been of considerable interest because several important classes of proteins, including the vesicle-targeting/fusion proteins known as SNAREs and the apoptosis-related proteins of the Bcl-2 family, among others, utilize this unique membrane-anchoring motif. Here, we use a bioinformatic technique to develop a comprehensive list of potentially tail-anchored proteins in the human genome. Our final list contains 411 entries derived from 325 unique genes. We also analyzed both known and predicted tail-anchored proteins with respect to the amino acid composition of the transmembrane segments. This analysis revealed a distinctive composition of the membrane anchor in SNARE proteins.
KW - Endoplasmic reticulum
KW - Membrane protein
KW - Mitochondrial outer membrane
KW - Protein targeting
KW - SNARE
UR - http://www.scopus.com/inward/record.url?scp=35948944937&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35948944937&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0854.2007.00661.x
DO - 10.1111/j.1600-0854.2007.00661.x
M3 - Article
C2 - 17892534
AN - SCOPUS:35948944937
SN - 1398-9219
VL - 8
SP - 1687
EP - 1694
JO - Traffic
JF - Traffic
IS - 12
ER -