A cell cycleregulated Toxoplasma deubiquitinase, TgOTUD3A, targets polyubiquitins with specific lysine linkages

Animesh Dhara, Anthony P. Sinai

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The contribution of ubiquitin-mediated mechanisms in the regulation of the Toxoplasma gondii cell cycle has remained largely unexplored. Here, we describe the functional characterization of a T. gondii deubiquitinase (TGGT1_258780) of the ovarian-tumor domain-containing (OTU) family, which, based on its structural homology to the human OTUD3 clade, has been designated TgOTUD3A. The TgOTUD3A protein is expressed in a cell cycle-dependent manner mimicking its mRNA expression, indicating that it is regulated primarily at the transcriptional level. TgOTUD3A, which was found in the cytoplasm at low levels in G1 parasites, increased in abundance with the progression of the cell cycle and exhibited partial localization to the developing daughter scaffolds during cytokinesis. Recombinant TgOTUD3A but not a catalytic-site mutant TgOTUD3A (C229A) exhibited activity against poly- but not monoubiquitinated targets. This activity was selective for polyubiquitin chains with preference for specific lysine linkages (K48 > K11 > K63). All three of these polyubiquitin linkage modifications were found to be present in Toxoplasma, where they exhibited differential levels and localization patterns in a cell cycle-dependent manner. TgOTUD3A removed ubiquitin from the K48- but not the K63-linked ubiquitinated T. gondii proteins independently of the modified target protein, thereby exhibiting the characteristics of an exodeubiquitinase. In addition to cell cycle association, the demonstration of multiple ubiquitin linkages together with the selective deubiquitinase activity of TgOTUD3A reveals an unappreciated level of complexity in the T. gondii "ubiquitin code."

Original languageEnglish
Article numbere00085-16
Issue number3
StatePublished - May 1 2016

Bibliographical note

Publisher Copyright:
© 2016 Dhara and Sinai.


  • Cell cycle
  • Deubiquitinase
  • OTU
  • Polyubiquitin
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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