We have previously reported that cholinergic neuron-specific expression of the human choline acetyltransferase gene is mediated by two co-operative silencers. We have now localized the proximal silencer to the region from nucleotide -2195 to -2409, which contains two distinct E boxes (CACCTG and CATGTG). Deletion or mutation of either of these E boxes results in a loss of silencer activity. There are specific nuclear proteins in adrenergic cells which bind to each of the two E boxes. However, nuclear proteins from cholinergic cells only bind the 5′ E box not the 3′ E box. It is this interaction which appears to be the cause of the inactivity of this silencer in these cells.
|Number of pages||9|
|Journal||Molecular Brain Research|
|State||Published - May 1995|
Bibliographical noteFunding Information:
This research was supported in part by a grant from the National Institute on Aging (AG05893) to L.B.H.
- Cholinergic expression
- Gene regulation
- Repressor element
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience