Abstract
We have previously reported that cholinergic neuron-specific expression of the human choline acetyltransferase gene is mediated by two co-operative silencers. We have now localized the proximal silencer to the region from nucleotide -2195 to -2409, which contains two distinct E boxes (CACCTG and CATGTG). Deletion or mutation of either of these E boxes results in a loss of silencer activity. There are specific nuclear proteins in adrenergic cells which bind to each of the two E boxes. However, nuclear proteins from cholinergic cells only bind the 5′ E box not the 3′ E box. It is this interaction which appears to be the cause of the inactivity of this silencer in these cells.
Original language | English |
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Pages (from-to) | 106-114 |
Number of pages | 9 |
Journal | Molecular Brain Research |
Volume | 30 |
Issue number | 1 |
DOIs | |
State | Published - May 1995 |
Bibliographical note
Funding Information:This research was supported in part by a grant from the National Institute on Aging (AG05893) to L.B.H.
Keywords
- Cholinergic expression
- Gene regulation
- Repressor element
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience