A cell type-specific silencer in the human choline acetyltransferase gene requiring two distinct and interactive E boxes

Yi Ping Li, Fred Baskin, Richard Davis, Donghai Wu, Louis B. Hersh

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We have previously reported that cholinergic neuron-specific expression of the human choline acetyltransferase gene is mediated by two co-operative silencers. We have now localized the proximal silencer to the region from nucleotide -2195 to -2409, which contains two distinct E boxes (CACCTG and CATGTG). Deletion or mutation of either of these E boxes results in a loss of silencer activity. There are specific nuclear proteins in adrenergic cells which bind to each of the two E boxes. However, nuclear proteins from cholinergic cells only bind the 5′ E box not the 3′ E box. It is this interaction which appears to be the cause of the inactivity of this silencer in these cells.

Original languageEnglish
Pages (from-to)106-114
Number of pages9
JournalMolecular Brain Research
Volume30
Issue number1
DOIs
StatePublished - May 1995

Bibliographical note

Funding Information:
This research was supported in part by a grant from the National Institute on Aging (AG05893) to L.B.H.

Keywords

  • Cholinergic expression
  • Gene regulation
  • Repressor element

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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