A cell type-specific silencer in the human choline acetyltransferase gene requiring two distinct and interactive E boxes

Yi Ping Li; Fred Baskin; Richard Davis; Donghai Wu; Louis B. Hersh

doi:10.1016/0169-328X(94)00285-M

A cell type-specific silencer in the human choline acetyltransferase gene requiring two distinct and interactive E boxes

Yi Ping Li, Fred Baskin, Richard Davis, Donghai Wu, Louis B. Hersh

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We have previously reported that cholinergic neuron-specific expression of the human choline acetyltransferase gene is mediated by two co-operative silencers. We have now localized the proximal silencer to the region from nucleotide -2195 to -2409, which contains two distinct E boxes (CACCTG and CATGTG). Deletion or mutation of either of these E boxes results in a loss of silencer activity. There are specific nuclear proteins in adrenergic cells which bind to each of the two E boxes. However, nuclear proteins from cholinergic cells only bind the 5′ E box not the 3′ E box. It is this interaction which appears to be the cause of the inactivity of this silencer in these cells.

Original language	English
Pages (from-to)	106-114
Number of pages	9
Journal	Molecular Brain Research
Volume	30
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(94)00285-M
State	Published - May 1995

Bibliographical note

Funding Information:
This research was supported in part by a grant from the National Institute on Aging (AG05893) to L.B.H.

Funding

This research was supported in part by a grant from the National Institute on Aging (AG05893) to L.B.H.

Funders	Funder number
National Institute on Aging	R55AG005893

Keywords

Cholinergic expression
Gene regulation
Repressor element

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/0169-328X(94)00285-M

Cite this

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title = "A cell type-specific silencer in the human choline acetyltransferase gene requiring two distinct and interactive E boxes",

abstract = "We have previously reported that cholinergic neuron-specific expression of the human choline acetyltransferase gene is mediated by two co-operative silencers. We have now localized the proximal silencer to the region from nucleotide -2195 to -2409, which contains two distinct E boxes (CACCTG and CATGTG). Deletion or mutation of either of these E boxes results in a loss of silencer activity. There are specific nuclear proteins in adrenergic cells which bind to each of the two E boxes. However, nuclear proteins from cholinergic cells only bind the 5′ E box not the 3′ E box. It is this interaction which appears to be the cause of the inactivity of this silencer in these cells.",

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note = "Funding Information: This research was supported in part by a grant from the National Institute on Aging (AG05893) to L.B.H.",

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AU - Baskin, Fred

AU - Davis, Richard

AU - Wu, Donghai

AU - Hersh, Louis B.

N1 - Funding Information: This research was supported in part by a grant from the National Institute on Aging (AG05893) to L.B.H.

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AB - We have previously reported that cholinergic neuron-specific expression of the human choline acetyltransferase gene is mediated by two co-operative silencers. We have now localized the proximal silencer to the region from nucleotide -2195 to -2409, which contains two distinct E boxes (CACCTG and CATGTG). Deletion or mutation of either of these E boxes results in a loss of silencer activity. There are specific nuclear proteins in adrenergic cells which bind to each of the two E boxes. However, nuclear proteins from cholinergic cells only bind the 5′ E box not the 3′ E box. It is this interaction which appears to be the cause of the inactivity of this silencer in these cells.

KW - Cholinergic expression

KW - Gene regulation

KW - Repressor element

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A cell type-specific silencer in the human choline acetyltransferase gene requiring two distinct and interactive E boxes

Abstract

Bibliographical note

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Keywords

ASJC Scopus subject areas

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