A chemical modification/interference study of yeast pre-mRNA spliceosome assembly and splicing.

B. C. Rymond, M. Rosbash

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

A chemical modification/interference assay was used to determine the yeast pre-mRNA sequence requirements for in vitro spliceosome assembly and splicing. Modifications of any of the nucleotides within the 5' splice site and branch point (TACTAAC box) consensus sequences as well as less conserved intron and exon positions were found to inhibit assembly and/or splicing. The interference pattern of the 5' splice site and TACTAAC box lesions increased as spliceosome assembly proceeded (complex III----complex I----complex II) and as splicing proceeded, suggesting that these sequence elements play multiple roles in the assembly of yeast spliceosomes and in the removal of intervening sequences. Furthermore, modification (or mutation) of a TACTAAC-like sequence upstream of the branch point was found to inhibit the rate of spliceosome assembly, implying a possible role for degenerate branch point sequences in modulating the efficiency of spliceosome assembly.

Original languageEnglish
Pages (from-to)428-439
Number of pages12
JournalGenes and Development
Volume2
Issue number4
DOIs
StatePublished - Apr 1988

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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