A Chemosensory Protein BtabCSP11 Mediates Reproduction in Bemisia tabaci

Yang Zeng; Austin Merchant; Qingjun Wu; Shaoli Wang; Lan Kong; Xuguo Zhou; Wen Xie; Youjun Zhang

doi:10.3389/fphys.2020.00709

A Chemosensory Protein BtabCSP11 Mediates Reproduction in Bemisia tabaci

Yang Zeng
, Austin Merchant
, Qingjun Wu
, Shaoli Wang
, Lan Kong
, Xuguo Zhou
, Wen Xie
, Youjun Zhang

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The olfactory system serves a vital role in the evolution and survival of insects, being involved in behaviors such as host seeking, foraging, mating, and oviposition. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the olfactory recognition process. In this study, BtabCSP11, a CSP11 gene from the whitefly Bemisia tabaci, was cloned and characterized. The open reading frame of BtabCSP11 encodes 136 amino acids, with four highly conserved cysteine residues. The temporal and spatial expression profiles showed that BtabCSP11 was highly expressed in the abdomens of B. tabaci females. Dietary RNA interference (RNAi)-based functional analysis showed substantially reduced fecundity in parthenogenetically reproduced females, suggesting a potential role of BtabCSP11 in B. tabaci reproduction. These combined results expand the function of CSPs beyond chemosensation.

Original language	English
Article number	709
Journal	Frontiers in Physiology
Volume	11
DOIs	https://doi.org/10.3389/fphys.2020.00709
State	Published - Jun 30 2020

Bibliographical note

Publisher Copyright:
© Copyright © 2020 Zeng, Merchant, Wu, Wang, Kong, Zhou, Xie and Zhang.

Funding

Funding. This research was supported by the National Key R&D Program of China (2019YFD1002100), the National Natural Science Foundation of China (31672032 and 31871970), China Agriculture Research System (CARS-24-C-02), the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables and the Science, and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS).

Funders	Funder number
Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables
CAAS-ASTIP-IVFCAAS
National Key Research and Development Program of China	2019YFD1002100
National Natural Science Foundation of China (NSFC)	31871970, 31672032
Chinese Academy of Agricultural Sciences
Agriculture Research System of China	CARS-24-C-02

Keywords

Bemisia tabaci
RNA interference
chemosensory proteins
expression profiles
reproduction

ASJC Scopus subject areas

Physiology
Physiology (medical)

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10.3389/fphys.2020.00709

Cite this

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title = "A Chemosensory Protein BtabCSP11 Mediates Reproduction in Bemisia tabaci",

abstract = "The olfactory system serves a vital role in the evolution and survival of insects, being involved in behaviors such as host seeking, foraging, mating, and oviposition. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the olfactory recognition process. In this study, BtabCSP11, a CSP11 gene from the whitefly Bemisia tabaci, was cloned and characterized. The open reading frame of BtabCSP11 encodes 136 amino acids, with four highly conserved cysteine residues. The temporal and spatial expression profiles showed that BtabCSP11 was highly expressed in the abdomens of B. tabaci females. Dietary RNA interference (RNAi)-based functional analysis showed substantially reduced fecundity in parthenogenetically reproduced females, suggesting a potential role of BtabCSP11 in B. tabaci reproduction. These combined results expand the function of CSPs beyond chemosensation.",

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author = "Yang Zeng and Austin Merchant and Qingjun Wu and Shaoli Wang and Lan Kong and Xuguo Zhou and Wen Xie and Youjun Zhang",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2020 Zeng, Merchant, Wu, Wang, Kong, Zhou, Xie and Zhang.",

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T1 - A Chemosensory Protein BtabCSP11 Mediates Reproduction in Bemisia tabaci

AU - Zeng, Yang

AU - Merchant, Austin

AU - Wu, Qingjun

AU - Wang, Shaoli

AU - Kong, Lan

AU - Zhou, Xuguo

AU - Xie, Wen

AU - Zhang, Youjun

PY - 2020/6/30

Y1 - 2020/6/30

N2 - The olfactory system serves a vital role in the evolution and survival of insects, being involved in behaviors such as host seeking, foraging, mating, and oviposition. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the olfactory recognition process. In this study, BtabCSP11, a CSP11 gene from the whitefly Bemisia tabaci, was cloned and characterized. The open reading frame of BtabCSP11 encodes 136 amino acids, with four highly conserved cysteine residues. The temporal and spatial expression profiles showed that BtabCSP11 was highly expressed in the abdomens of B. tabaci females. Dietary RNA interference (RNAi)-based functional analysis showed substantially reduced fecundity in parthenogenetically reproduced females, suggesting a potential role of BtabCSP11 in B. tabaci reproduction. These combined results expand the function of CSPs beyond chemosensation.

AB - The olfactory system serves a vital role in the evolution and survival of insects, being involved in behaviors such as host seeking, foraging, mating, and oviposition. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the olfactory recognition process. In this study, BtabCSP11, a CSP11 gene from the whitefly Bemisia tabaci, was cloned and characterized. The open reading frame of BtabCSP11 encodes 136 amino acids, with four highly conserved cysteine residues. The temporal and spatial expression profiles showed that BtabCSP11 was highly expressed in the abdomens of B. tabaci females. Dietary RNA interference (RNAi)-based functional analysis showed substantially reduced fecundity in parthenogenetically reproduced females, suggesting a potential role of BtabCSP11 in B. tabaci reproduction. These combined results expand the function of CSPs beyond chemosensation.

KW - Bemisia tabaci

KW - RNA interference

KW - chemosensory proteins

KW - expression profiles

KW - reproduction

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A Chemosensory Protein BtabCSP11 Mediates Reproduction in Bemisia tabaci

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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