The axolotl (Ambystoma mexicanum) provides critical models for studying regeneration, evolution, and development. However, its large genome (~32 Gb) presents a formidable barrier to genetic analyses. Recent efforts have yielded genome assemblies consisting of thousands of unordered scaffolds that resolve gene structures, but do not yet permit large-scale analyses of genome structure and function. We adapted an established mapping approach to leverage dense SNP typing information and for the first time assemble the axolotl genome into 14 chromosomes. Moreover, we used fluorescence in situ hybridization to verify the structure of these 14 scaffolds and assign each to its corresponding physical chromosome. This new assembly covers 27.3 Gb and encompasses 94% of annotated gene models on chromosomal scaffolds. We show the assembly's utility by resolving genome-wide orthologies between the axolotl and other vertebrates, identifying the footprints of historical introgression events that occurred during the development of axolotl genetic stocks, and precisely mapping several phenotypes including a large deletion underlying the cardiac mutant. This chromosome-scale assembly will greatly facilitate studies of the axolotl in biological research.
|Number of pages||8|
|State||Published - Feb 2019|
Bibliographical noteFunding Information:
This work was funded by grants from the National Institutes of Health (NIH) (R24OD010435) and Department of Defense (DOD) (W911NF1110475) to S.R.V. Animals used in this study were provided by the Ambystoma Genetic Stock Center, which is currently funded by the NIH (P40OD019794) and previously by the National Science Foundation (NSF) (DBI-0951484) to S.R.V. The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of NIH, DOD, or NSF. Partial computational support was provided by The University of Kentucky High Performance Computing complex.
© 2019 Smith et al.
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