A color segmentation-based method to quantify atherosclerotic lesion compositions with immunostaining

Congqing Wu; Alan Daugherty; Hong Lu

doi:10.1007/978-1-4939-7030-8_2

A color segmentation-based method to quantify atherosclerotic lesion compositions with immunostaining

Congqing Wu, Alan Daugherty, Hong Lu

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	21-30
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7030-8_2
State	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1614
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
Congqing Wu is supported by an American Heart Association Postdoctoral fellow award (16POST31140008). The authors' research work is supported by an Institutional Development Award from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20 GM103527 and R01 under grant numbers HL107319 and HL133723 from the National Institutes of Health of the United States of America. The content in this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

Antibody
Atherosclerosis
Color
Imaging
Immunostaining

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-7030-8_2

Cite this

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abstract = "There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.",

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AU - Lu, Hong

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N2 - There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.

AB - There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.

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KW - Imaging

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A color segmentation-based method to quantify atherosclerotic lesion compositions with immunostaining

Abstract

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Bibliographical note

Keywords

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