A color segmentation-based method to quantify atherosclerotic lesion compositions with immunostaining

Congqing Wu, Alan Daugherty, Hong Lu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations


There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages10
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
Congqing Wu is supported by an American Heart Association Postdoctoral fellow award (16POST31140008). The authors' research work is supported by an Institutional Development Award from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20 GM103527 and R01 under grant numbers HL107319 and HL133723 from the National Institutes of Health of the United States of America. The content in this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.


  • Antibody
  • Atherosclerosis
  • Color
  • Imaging
  • Immunostaining

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'A color segmentation-based method to quantify atherosclerotic lesion compositions with immunostaining'. Together they form a unique fingerprint.

Cite this