A color segmentation-based method to quantify atherosclerotic lesion compositions with immunostaining

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations

Abstract

There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages21-30
Number of pages10
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1614
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

  • Antibody
  • Atherosclerosis
  • Color
  • Imaging
  • Immunostaining

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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