TY - JOUR
T1 - A combined computational-experimental approach predicts human microRNA targets
AU - Kiriakidou, Marianthi
AU - Nelson, Peter T.
AU - Kouranov, Andrei
AU - Fitziev, Petko
AU - Bouyioukos, Costas
AU - Mourelatos, Zissimos
AU - Hatzigeorgiou, Artemis
PY - 2004/5/15
Y1 - 2004/5/15
N2 - A new paradigm of gene expression regulation has emerged recently with the discovery of microRNAs (miRNAs). Most, if not all, miRNAs are thought to control gene expression, mostly by base pairing with miRNA-recognition elements (MREs) found in their messenger RNA (mRNA) targets. Although a large number of human miRNAs have been reported, many of their mRNA targets remain unknown. Here we used a combined bioinformatics and experimental approach to identify important rules governing miRNA-MRE recognition that allow prediction of human miRNA targets. We describe a computational program, "DIANA-microT", that identifies mRNA targets for animal miRNAs and predicts mRNA targets, bearing single MREs, for human and mouse miRNAs.
AB - A new paradigm of gene expression regulation has emerged recently with the discovery of microRNAs (miRNAs). Most, if not all, miRNAs are thought to control gene expression, mostly by base pairing with miRNA-recognition elements (MREs) found in their messenger RNA (mRNA) targets. Although a large number of human miRNAs have been reported, many of their mRNA targets remain unknown. Here we used a combined bioinformatics and experimental approach to identify important rules governing miRNA-MRE recognition that allow prediction of human miRNA targets. We describe a computational program, "DIANA-microT", that identifies mRNA targets for animal miRNAs and predicts mRNA targets, bearing single MREs, for human and mouse miRNAs.
KW - Argonaute
KW - Bioinformatics
KW - MicroRNA
KW - MicroRNA targets
KW - MicroRNP
KW - RNAi
UR - http://www.scopus.com/inward/record.url?scp=2442672918&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442672918&partnerID=8YFLogxK
U2 - 10.1101/gad.1184704
DO - 10.1101/gad.1184704
M3 - Article
C2 - 15131085
AN - SCOPUS:2442672918
SN - 0890-9369
VL - 18
SP - 1165
EP - 1178
JO - Genes and Development
JF - Genes and Development
IS - 10
ER -