A comparative study of carbon fiber-based microelectrodes for the measurement of nitric oxide in brain tissue

Ricardo M. Santos; Cátia F. Lourenço; Ana P. Piedade; Rodney Andrews; François Pomerleau; Peter Huettl; Greg A. Gerhardt; João Laranjinha; Rui M. Barbosa

doi:10.1016/j.bios.2008.06.034

A comparative study of carbon fiber-based microelectrodes for the measurement of nitric oxide in brain tissue

Ricardo M. Santos, Cátia F. Lourenço, Ana P. Piedade, Rodney Andrews, François Pomerleau, Peter Huettl, Greg A. Gerhardt, João Laranjinha, Rui M. Barbosa

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

The measurement of Nitric oxide ({radical dot}NO) in real-time has been a major concern due to the involvement of this ubiquitous free radical modulator in several physiological and pathological pathways in tissues. Here we performed a study aiming at evaluating different types of carbon fibers, namely Textron, Amoco, Courtaulds and carbon nanotubes (University of Kentucky) covered with Nafion^®/o-phenylenediamine (o-PD) for {radical dot}NO measurement in terms of sensitivity, LOD, response time and selectivity against major potential interferents in the brain (ascorbate, nitrite and dopamine). The results indicate that, as compared with the other carbon fibers and nanotubes, Textron carbon fiber microelectrodes coated with two layers of Nafion^® and o-PD exhibited better characteristics for {radical dot}NO measurement as they are highly selective against ascorbate (>30,000:1), nitrite (>2000:1) and dopamine (>80:1). These coated Textron microelectrodes showed an average sensitivity of 341 ± 120 pA/μM and a detection limit of 16 ± 11 nM. The better performance of the Textron fibers is likely related to a stronger adhesion or more uniform coating of the Nafion^® and o-PD polymers to the fiber surface. In addition, the background current of the Textron carbon fibers is low, contributing to the excellent signal-to-noise for detection of {radical dot}NO.

Original language	English
Pages (from-to)	704-709
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/j.bios.2008.06.034
State	Published - Dec 1 2008

Bibliographical note

Funding Information:
AP acknowledges financial support to Fundação para a Ciência e a Tecnologia (SFRH/BPD/27139/2006).

Funding Information:
This work was supported by a grant from Fundação para a Ciência e Tecnologia PTDC/AGR-ALI/71262/2006.

Funding Information:
RA acknowledges the financial support of the U.S. Department of Energy, under award no. DE-FC26-03NT41874. However, any opinions, findings, conclusions, or recommendations expressed herein are those of the authors and do not necessarily reflect the views of the DOE.

Funding

AP acknowledges financial support to Fundação para a Ciência e a Tecnologia (SFRH/BPD/27139/2006). This work was supported by a grant from Fundação para a Ciência e Tecnologia PTDC/AGR-ALI/71262/2006. RA acknowledges the financial support of the U.S. Department of Energy, under award no. DE-FC26-03NT41874. However, any opinions, findings, conclusions, or recommendations expressed herein are those of the authors and do not necessarily reflect the views of the DOE.

Funders	Funder number
Michigan State University-U.S. Department of Energy (MSU-DOE) Plant Research Laboratory	DE-FC26-03NT41874
Instituto Nacional de Ciência e Tecnologia para Excitotoxicidade e Neuroproteção	PTDC/AGR-ALI/71262/2006, SFRH/BPD/27139/2006

Keywords

Carbon fiber
Carbon nanotubes
Microelectrode
Nafion
Nitric oxide
o-phenylenediamine

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2008.06.034

Cite this

@article{4051824731fd4b1695d6953c8225cb2d,

title = "A comparative study of carbon fiber-based microelectrodes for the measurement of nitric oxide in brain tissue",

abstract = "The measurement of Nitric oxide ({radical dot}NO) in real-time has been a major concern due to the involvement of this ubiquitous free radical modulator in several physiological and pathological pathways in tissues. Here we performed a study aiming at evaluating different types of carbon fibers, namely Textron, Amoco, Courtaulds and carbon nanotubes (University of Kentucky) covered with Nafion{\textregistered}/o-phenylenediamine (o-PD) for {radical dot}NO measurement in terms of sensitivity, LOD, response time and selectivity against major potential interferents in the brain (ascorbate, nitrite and dopamine). The results indicate that, as compared with the other carbon fibers and nanotubes, Textron carbon fiber microelectrodes coated with two layers of Nafion{\textregistered} and o-PD exhibited better characteristics for {radical dot}NO measurement as they are highly selective against ascorbate (>30,000:1), nitrite (>2000:1) and dopamine (>80:1). These coated Textron microelectrodes showed an average sensitivity of 341 ± 120 pA/μM and a detection limit of 16 ± 11 nM. The better performance of the Textron fibers is likely related to a stronger adhesion or more uniform coating of the Nafion{\textregistered} and o-PD polymers to the fiber surface. In addition, the background current of the Textron carbon fibers is low, contributing to the excellent signal-to-noise for detection of {radical dot}NO.",

keywords = "Carbon fiber, Carbon nanotubes, Microelectrode, Nafion, Nitric oxide, o-phenylenediamine",

author = "Santos, {Ricardo M.} and Louren{\c c}o, {C{\'a}tia F.} and Piedade, {Ana P.} and Rodney Andrews and Fran{\c c}ois Pomerleau and Peter Huettl and Gerhardt, {Greg A.} and Jo{\~a}o Laranjinha and Barbosa, {Rui M.}",

note = "Funding Information: AP acknowledges financial support to Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (SFRH/BPD/27139/2006). Funding Information: This work was supported by a grant from Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia PTDC/AGR-ALI/71262/2006. Funding Information: RA acknowledges the financial support of the U.S. Department of Energy, under award no. DE-FC26-03NT41874. However, any opinions, findings, conclusions, or recommendations expressed herein are those of the authors and do not necessarily reflect the views of the DOE.",

year = "2008",

month = dec,

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doi = "10.1016/j.bios.2008.06.034",

language = "English",

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T1 - A comparative study of carbon fiber-based microelectrodes for the measurement of nitric oxide in brain tissue

AU - Santos, Ricardo M.

AU - Lourenço, Cátia F.

AU - Piedade, Ana P.

AU - Andrews, Rodney

AU - Pomerleau, François

AU - Huettl, Peter

AU - Gerhardt, Greg A.

AU - Laranjinha, João

AU - Barbosa, Rui M.

N1 - Funding Information: AP acknowledges financial support to Fundação para a Ciência e a Tecnologia (SFRH/BPD/27139/2006). Funding Information: This work was supported by a grant from Fundação para a Ciência e Tecnologia PTDC/AGR-ALI/71262/2006. Funding Information: RA acknowledges the financial support of the U.S. Department of Energy, under award no. DE-FC26-03NT41874. However, any opinions, findings, conclusions, or recommendations expressed herein are those of the authors and do not necessarily reflect the views of the DOE.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - The measurement of Nitric oxide ({radical dot}NO) in real-time has been a major concern due to the involvement of this ubiquitous free radical modulator in several physiological and pathological pathways in tissues. Here we performed a study aiming at evaluating different types of carbon fibers, namely Textron, Amoco, Courtaulds and carbon nanotubes (University of Kentucky) covered with Nafion®/o-phenylenediamine (o-PD) for {radical dot}NO measurement in terms of sensitivity, LOD, response time and selectivity against major potential interferents in the brain (ascorbate, nitrite and dopamine). The results indicate that, as compared with the other carbon fibers and nanotubes, Textron carbon fiber microelectrodes coated with two layers of Nafion® and o-PD exhibited better characteristics for {radical dot}NO measurement as they are highly selective against ascorbate (>30,000:1), nitrite (>2000:1) and dopamine (>80:1). These coated Textron microelectrodes showed an average sensitivity of 341 ± 120 pA/μM and a detection limit of 16 ± 11 nM. The better performance of the Textron fibers is likely related to a stronger adhesion or more uniform coating of the Nafion® and o-PD polymers to the fiber surface. In addition, the background current of the Textron carbon fibers is low, contributing to the excellent signal-to-noise for detection of {radical dot}NO.

AB - The measurement of Nitric oxide ({radical dot}NO) in real-time has been a major concern due to the involvement of this ubiquitous free radical modulator in several physiological and pathological pathways in tissues. Here we performed a study aiming at evaluating different types of carbon fibers, namely Textron, Amoco, Courtaulds and carbon nanotubes (University of Kentucky) covered with Nafion®/o-phenylenediamine (o-PD) for {radical dot}NO measurement in terms of sensitivity, LOD, response time and selectivity against major potential interferents in the brain (ascorbate, nitrite and dopamine). The results indicate that, as compared with the other carbon fibers and nanotubes, Textron carbon fiber microelectrodes coated with two layers of Nafion® and o-PD exhibited better characteristics for {radical dot}NO measurement as they are highly selective against ascorbate (>30,000:1), nitrite (>2000:1) and dopamine (>80:1). These coated Textron microelectrodes showed an average sensitivity of 341 ± 120 pA/μM and a detection limit of 16 ± 11 nM. The better performance of the Textron fibers is likely related to a stronger adhesion or more uniform coating of the Nafion® and o-PD polymers to the fiber surface. In addition, the background current of the Textron carbon fibers is low, contributing to the excellent signal-to-noise for detection of {radical dot}NO.

KW - Carbon fiber

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KW - Microelectrode

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KW - Nitric oxide

KW - o-phenylenediamine

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A comparative study of carbon fiber-based microelectrodes for the measurement of nitric oxide in brain tissue

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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