A comparison of pro-inflammatory cytokine mRNA expression in equine bronchoalveolar lavage (BAL) and peripheral blood

S. Hansen; K. E. Baptiste; J. Fjeldborg; A. Betancourt; D. W. Horohov

doi:10.1016/j.vetimm.2014.02.001

A comparison of pro-inflammatory cytokine mRNA expression in equine bronchoalveolar lavage (BAL) and peripheral blood

S. Hansen, K. E. Baptiste, J. Fjeldborg, A. Betancourt, D. W. Horohov

Veterinary Science

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

An imbalance in various cytokine mRNA expression in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) in horses suffering from recurrent airway obstruction (RAO) has been demonstrated; however, the natural variation of cytokine expression in healthy horses has yet to be described.The objectives of this study were to: (1) identify age-related differences in the expression of pro-inflammatory cytokines in BAL and PBMC, (2) compare the expression of pro-inflammatory cytokines between BAL and PBMC, and (3) evaluate the relationship between BAL pro-inflammatory cytokine expression and BAL cytology.Cells from BAL and PBMC were collected from 66 horses and stimulated with LPS and PGN. The expression of IL-1β, IL-6, IL-8, TNF-α and TLR-4 were measured using qPCR.A significant age-related decrease for IL-1β and IL-8 in BAL, significant difference in cytokine mRNA expressions between BAL and PBMC and a significant relationship between TLR-4 and IL-8 in LPS stimulated BAL samples and the percentage of neutrophils in BAL cytology were found.In conclusion, the natural age-related decrease in pro-inflammatory cytokine expression in BAL is new knowledge; this in contrast to other age-related findings associated with PBMC, where pro-inflammatory cytokines are known to increase with age in both horses and humans, the phenomenon known as 'inflamm-aging'.

Original language	English
Pages (from-to)	238-243
Number of pages	6
Journal	Veterinary Immunology and Immunopathology
Volume	158
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetimm.2014.02.001
State	Published - Apr 15 2014

Bibliographical note

Funding Information:
S. Hansen was funded by a Danish Government PhD grant . Additional funding was generously provided by the following Danish funds: Familien Hede Nielsens Foundation , Toosbuys Foundation , Viktor A Goldschmidts foundation , Christian and Ottilia Brorsons travel fund and the Kustos foundation of 1881 . The authors are grateful to Tina Roust and Maria Rhod, laboratory technicians and students at the University Hospital for Large Animals, Faculty of Life Science, University of Copenhagen for assistance in collection and processing samples from the horses.

Keywords

Aging
BAL
Equine
Polymerase chain reaction
Pro-inflammatory cytokines

ASJC Scopus subject areas

Immunology
General Veterinary

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10.1016/j.vetimm.2014.02.001

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title = "A comparison of pro-inflammatory cytokine mRNA expression in equine bronchoalveolar lavage (BAL) and peripheral blood",

abstract = "An imbalance in various cytokine mRNA expression in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) in horses suffering from recurrent airway obstruction (RAO) has been demonstrated; however, the natural variation of cytokine expression in healthy horses has yet to be described.The objectives of this study were to: (1) identify age-related differences in the expression of pro-inflammatory cytokines in BAL and PBMC, (2) compare the expression of pro-inflammatory cytokines between BAL and PBMC, and (3) evaluate the relationship between BAL pro-inflammatory cytokine expression and BAL cytology.Cells from BAL and PBMC were collected from 66 horses and stimulated with LPS and PGN. The expression of IL-1β, IL-6, IL-8, TNF-α and TLR-4 were measured using qPCR.A significant age-related decrease for IL-1β and IL-8 in BAL, significant difference in cytokine mRNA expressions between BAL and PBMC and a significant relationship between TLR-4 and IL-8 in LPS stimulated BAL samples and the percentage of neutrophils in BAL cytology were found.In conclusion, the natural age-related decrease in pro-inflammatory cytokine expression in BAL is new knowledge; this in contrast to other age-related findings associated with PBMC, where pro-inflammatory cytokines are known to increase with age in both horses and humans, the phenomenon known as 'inflamm-aging'.",

keywords = "Aging, BAL, Equine, Polymerase chain reaction, Pro-inflammatory cytokines",

author = "S. Hansen and Baptiste, {K. E.} and J. Fjeldborg and A. Betancourt and Horohov, {D. W.}",

note = "Funding Information: S. Hansen was funded by a Danish Government PhD grant . Additional funding was generously provided by the following Danish funds: Familien Hede Nielsens Foundation , Toosbuys Foundation , Viktor A Goldschmidts foundation , Christian and Ottilia Brorsons travel fund and the Kustos foundation of 1881 . The authors are grateful to Tina Roust and Maria Rhod, laboratory technicians and students at the University Hospital for Large Animals, Faculty of Life Science, University of Copenhagen for assistance in collection and processing samples from the horses.",

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AU - Hansen, S.

AU - Baptiste, K. E.

AU - Fjeldborg, J.

AU - Betancourt, A.

AU - Horohov, D. W.

N1 - Funding Information: S. Hansen was funded by a Danish Government PhD grant . Additional funding was generously provided by the following Danish funds: Familien Hede Nielsens Foundation , Toosbuys Foundation , Viktor A Goldschmidts foundation , Christian and Ottilia Brorsons travel fund and the Kustos foundation of 1881 . The authors are grateful to Tina Roust and Maria Rhod, laboratory technicians and students at the University Hospital for Large Animals, Faculty of Life Science, University of Copenhagen for assistance in collection and processing samples from the horses.

PY - 2014/4/15

Y1 - 2014/4/15

N2 - An imbalance in various cytokine mRNA expression in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) in horses suffering from recurrent airway obstruction (RAO) has been demonstrated; however, the natural variation of cytokine expression in healthy horses has yet to be described.The objectives of this study were to: (1) identify age-related differences in the expression of pro-inflammatory cytokines in BAL and PBMC, (2) compare the expression of pro-inflammatory cytokines between BAL and PBMC, and (3) evaluate the relationship between BAL pro-inflammatory cytokine expression and BAL cytology.Cells from BAL and PBMC were collected from 66 horses and stimulated with LPS and PGN. The expression of IL-1β, IL-6, IL-8, TNF-α and TLR-4 were measured using qPCR.A significant age-related decrease for IL-1β and IL-8 in BAL, significant difference in cytokine mRNA expressions between BAL and PBMC and a significant relationship between TLR-4 and IL-8 in LPS stimulated BAL samples and the percentage of neutrophils in BAL cytology were found.In conclusion, the natural age-related decrease in pro-inflammatory cytokine expression in BAL is new knowledge; this in contrast to other age-related findings associated with PBMC, where pro-inflammatory cytokines are known to increase with age in both horses and humans, the phenomenon known as 'inflamm-aging'.

AB - An imbalance in various cytokine mRNA expression in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) in horses suffering from recurrent airway obstruction (RAO) has been demonstrated; however, the natural variation of cytokine expression in healthy horses has yet to be described.The objectives of this study were to: (1) identify age-related differences in the expression of pro-inflammatory cytokines in BAL and PBMC, (2) compare the expression of pro-inflammatory cytokines between BAL and PBMC, and (3) evaluate the relationship between BAL pro-inflammatory cytokine expression and BAL cytology.Cells from BAL and PBMC were collected from 66 horses and stimulated with LPS and PGN. The expression of IL-1β, IL-6, IL-8, TNF-α and TLR-4 were measured using qPCR.A significant age-related decrease for IL-1β and IL-8 in BAL, significant difference in cytokine mRNA expressions between BAL and PBMC and a significant relationship between TLR-4 and IL-8 in LPS stimulated BAL samples and the percentage of neutrophils in BAL cytology were found.In conclusion, the natural age-related decrease in pro-inflammatory cytokine expression in BAL is new knowledge; this in contrast to other age-related findings associated with PBMC, where pro-inflammatory cytokines are known to increase with age in both horses and humans, the phenomenon known as 'inflamm-aging'.

KW - Aging

KW - BAL

KW - Equine

KW - Polymerase chain reaction

KW - Pro-inflammatory cytokines

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SN - 0165-2427

VL - 158

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EP - 243

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 3-4

ER -

A comparison of pro-inflammatory cytokine mRNA expression in equine bronchoalveolar lavage (BAL) and peripheral blood

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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